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HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 5:95)

HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 5:95) application for HPLC

Materials

analytical column

Product No.
Description
Pricing

53981-U

Ascentis® Express HILIC, 2.7 μm HPLC Column

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

standard

Product No.
Description
Pricing

C7252

D-(+)-Cellobiose

≥98% (HPLC)

A3256

L-(+)-Arabinose

≥99% (GC)

84097

Sucrose

BioUltra, for molecular biology, ≥99.5% (HPLC)

1287700

Galactose

United States Pharmacopeia (USP) Reference Standard

G0350500

Glucose

European Pharmacopoeia (EP) Reference Standard

X0200000

Xylose

European Pharmacopoeia (EP) Reference Standard

1375182

Mannose

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] water; [B] acetonitrile; (5:95, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

Description

Analysis Note

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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