Skip to Content
Merck
  • Pesticide determination in rose petals using dispersive solid-phase extraction followed by gas chromatography-tandem mass spectrometry.

Pesticide determination in rose petals using dispersive solid-phase extraction followed by gas chromatography-tandem mass spectrometry.

Analytical and bioanalytical chemistry (2014-10-27)
Oriane Tascone, Marina Shirshikova, Céline Roy, Uwe J Meierhenrich
ABSTRACT

Damascena and centifolia roses are cultivated worldwide for their petal extracts that contain key odorant ingredients of perfumes. The analytical identification and quantification of pesticides in rose petals have never been described in the literature. Here, we report on a newly developed method using dispersive solid-phase extraction (d-SPE) cleanup followed by gas chromatography-tandem mass spectrometry for the quantitative determination of multi-residue pesticides in rose petals. Analytes were extracted from the matrix using acetonitrile and a mixture of salts containing magnesium sulfate, sodium citrate, sodium chloride, and sodium sesquihydrate. Samples were cleaned up twice by d-SPE applying primary and secondary amines (PSAs), magnesium sulfate, C18, and graphitized carbon black (GCB). Two fortification levels of 0.05 and 0.5 mg kg(-1) were assessed for method validation purposes. The obtained pesticide recoveries were in the range of 70-120 % with a relative standard deviation (RSD) of less than 20 %. The newly developed method was allowed for the quantification of 57 pesticides residues. It was applied to pesticide residue detection in rose petals from an organic field, without treatment, compared to those from a field with classic phytosanitary treatment using fungicide and/or insecticide. We did not detect pesticide residues in rose petals from the organic field. The classically treated samples of roses contained pesticides such as chlorpyriphos and methidathion which are in accordance with the previous application of these pesticides on the roses. Insecticides were quantified at 0.05 mg kg(-1) rose petal maximum.

MATERIALS
Product Number
Brand
Product Description

Supelco
Acetic acid, analytical standard
Sigma-Aldrich
Sodium chloride, BioUltra, for molecular biology, ≥99.5% (AT)
Sigma-Aldrich
Sodium chloride solution, 0.85%
Sigma-Aldrich
Linalool, 97%
Supelco
(−)-β-Citronellol, analytical standard
Sigma-Aldrich
Citrate Concentrated Solution, BioUltra, for molecular biology, 1 M in H2O
Sigma-Aldrich
Sodium chloride, random crystals, optical grade, 99.9% trace metals basis
Supelco
Eugenol, PESTANAL®, analytical standard
Supelco
Sodium chloride, reference material for titrimetry, certified by BAM, >99.5%
Sigma-Aldrich
Sodium chloride, 99.999% trace metals basis
Sigma-Aldrich
Geraniol, 98%
Sigma-Aldrich
Eugenol, ReagentPlus®, 99%
Sigma-Aldrich
Linalool, ≥97%, FCC, FG
Sigma-Aldrich
Acetic acid, for luminescence, BioUltra, ≥99.5% (GC)
Sigma-Aldrich
Sodium chloride-35Cl, 99 atom % 35Cl
Sigma-Aldrich
Acetic acid, ≥99.5%, FCC, FG
Sigma-Aldrich
Sodium chloride solution, 5 M
Sigma-Aldrich
Sodium chloride solution, 0.9% in water, BioXtra, suitable for cell culture
Sigma-Aldrich
Sodium chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
Sigma-Aldrich
Sodium chloride, for molecular biology, DNase, RNase, and protease, none detected, ≥99% (titration)
Sigma-Aldrich
Sodium chloride, BioXtra, ≥99.5% (AT)
Sigma-Aldrich
Citrate Concentrated Solution, BioReagent, suitable for coagulation assays, 4 % (w/v)
Supelco
Eugenol, certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland
Sigma-Aldrich
Carbon, mesoporous, hydrophilic pore surface
Sigma-Aldrich
Sodium chloride, BioPerformance Certified, ≥99% (titration), suitable for insect cell culture, suitable for plant cell culture
Sigma-Aldrich
Citronellyl acetate, ≥95%, FCC, FG
Sigma-Aldrich
Acetic acid-12C2, 99.9 atom % 12C
Sigma-Aldrich
Carbon, mesoporous, nanopowder, graphitized, less than 250 ppm Al, Ti, Fe, Ni, Cu, and Zn combined
Sigma-Aldrich
Sodium chloride, tested according to Ph. Eur.
Supelco
Linalool, analytical standard