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SRP0171

Sigma-Aldrich

Jak2 Jh1 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

Synonym(s):

JAK2, JTK10, Janus kinase 2, Just another kinase 2, Tyrosine-protein kinase JAK2

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in baculovirus infected insect cells

Assay

≥80% (SDS-PAGE)

form

aqueous solution

mol wt

42.5 kDa

packaging

pkg of 10 μg

concentration

>0.02 mg/mL

NCBI accession no.

NM_004972

UniProt accession no.

O60674

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... JAK2(3717)

General description

Human Jak2 (GenBank Accession No. NM_004972), (amino acids. 808-end) with N-terminal His tag, MW = 42.5 kDa, expressed in Baculovirus expression system.

Application

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Unit Definition

One unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Tyr substrate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 10% glycerol and 1 mM DTT.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kamonpan Sanachai et al.
RSC medicinal chemistry, 12(3), 430-438 (2021-05-29)
The Janus kinase (JAK) and epidermal growth factor receptor (EGFR) have been considered as potential targets for cancer therapy due to their role in regulating proliferation and survival of cancer cells. In the present study, the aromatic alkyl-amino analogs of
Kamonpan Sanachai et al.
ACS omega, 7(37), 33548-33559 (2022-09-27)
Janus kinases (JAKs) are nonreceptor protein tyrosine kinases that play a role in a broad range of cell signaling. JAK2 and JAK3 have been involved in the pathogenesis of common lymphoid-derived diseases and leukemia cancer. Thus, inhibition of both JAK2
Kamonpan Sanachai et al.
ACS omega, 7(37), 33587-33598 (2022-09-27)
Janus kinases (JAKs) are involved in a wide variety of cell signaling associated with T-cell and B-cell mediated diseases. The pathogenesis of common lymphoid-derived diseases and leukemia cancer has been implicated in JAK2 and JAK3. Therefore, to decrease the risk
Kamonpan Sanachai et al.
ACS omega, 5(1), 369-377 (2020-01-21)
Janus kinases (JAKs) are enzymes involved in signaling pathways that affect hematopoiesis and immune cell functions. JAK1, JAK2, and JAK3 play different roles in numerous diseases of the immune system and have also been considered as potential targets for cancer
Chalita Washington et al.
PLoS genetics, 16(6), e1008715-e1008715 (2020-06-20)
Dysregulation of the Ras oncogene in development causes developmental disorders, "Rasopathies," whereas mutational activation or amplification of Ras in differentiated tissues causes cancer. Rabex-5 (also called RabGEF1) inhibits Ras by promoting Ras mono- and di-ubiquitination. We report here that Rabex-5-mediated

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