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O2260

Sigma-Aldrich

Mouse Osteopontin

recombinant, expressed in NSO cells, powder, suitable for cell culture

Synonym(s):

Bone Sialoprotein-1, Early T Lymphocyte Activation Protein-1 (ETA-1), OPN, Secreted Phosphoprotein 1 (Spp1)

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About This Item

MDL number:
MFCD03100698
UNSPSC Code:
12352202
NACRES:
NA.32

product name

Osteopontin from mouse, >95% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder, suitable for cell culture

biological source

mouse

Quality Level

100

recombinant

expressed in NSO cells

sterility

sterile

Assay

>95% (SDS-PAGE)

form

lyophilized powder

mol wt

30 kDa by SDS-PAGE (reducing)
 65 kDa by SDS-PAGE (reducing) (doublet)

packaging

pkg of 50 μg

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

UniProt accession no.

P10923

shipped in

dry ice

storage temp.

−20°C

Gene Information

mouse ... Spp1(20750)

Application

Osteopontin from mouse has been used:
  • to study its role in a mouse model of preterm brain injury
  • as a positive control to analyze the relationship between the vascular calcification process and osteopontin (OPN) overexpression in the neointima of atherosclerotic plaques
  • to study its interaction on the crystal growth process of hydroxyapatite in agarose hydrogels

Biochem/physiol Actions

Osteopontin (OPN) is a secreted, highly acidic, calcium binding, phosphorylated glycoprotein that is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. In activated macrophages, OPN inhibits nitric oxide production and cytotoxicity. Human, mouse, rat, pig and bovine OPN share 40% amino acid sequence identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 2.5 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to mediate 293 cell adhesion.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Osteopontin upregulation in atherogenesis is associated with cellular oxidative stress triggered by the activation of scavenger receptors
Jimenez CAE, et al.
Archives of Medical Research, 43(2), 102-111 (2012)
Osteopontin as a means to cope with environmental insults: regulation of inflammation, tissue remodeling, and cell survival
Denhardt DT, et al.
The Journal of Clinical Investigation, 107(9), 1055-1061 (2001)
Effect of osteopontin, chondroitin sulfates (B, C), and human serum albumin on the crystallization behavior of hydroxyapatite in agarose and silica hydrogels
Jimenez CAE, et al.
Crystal Growth & Design, 8(4), 1335-1339 (2008)
The effect of osteopontin and osteopontin-derived peptides on preterm brain injury
Albertsson AM, et al.
Journal of Neuroinflammation, 11(1), 197-197 (2014)
Xia Ying et al.
Oncology reports, 32(4), 1550-1556 (2014-08-12)
Osteopontin (OPN) is a secreted phosphorylated and glycosylated protein, which plays an important role in carcinogenesis and metastasis. In hepatocellular carcinoma (HCC), OPN is being investigated either as a therapeutic target gene or as a biomarker for diagnosis. Yet, the
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