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Key Documents

M3694

Sigma-Aldrich

Anti-α1,2-Mannosidase IA antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-HUMM3, Anti-HUMM9, Anti-MAN9

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About This Item

MDL number:
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~73 kDa

species reactivity

human, rat, mouse

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human HepG2 cells or rat NRK cells
western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of mouse NIH3T3 cells

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MAN1A1(4121)
mouse ... Man1a(17155)
rat ... Man1a1(294410)

General description

α1,2-Mannosidase IA is a type II transmembrane Golgi-resident enzyme that belongs to class I α1,2-Mannosidases (glycosylhydrolase family 47). The highly conserved Class I α1,2-Mannosidases classified into three subgroups according to their enzymatic activities. α1,2-Mannosidase IA is predominantly detected in the juxtanuclear Golgi region. The three subgroups differ in their expression pattern, subcellular localization, and substrate specificity.

Immunogen

synthetic peptide corresponding to amino acids 138-154 of human α1,2-mannosidase IA with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse.

Application

Anti-α1,2-Mannosidase IA antibody produced in rabbit has been used in:
  • immunohistochemistry
  • immunoblotting
  • immunofluorescence

Biochem/physiol Actions

α1,2-Mannosidase IA belongs to the first subgroup out of the three subgroups of class I α1,2-Mannosidases. The first subgroup consists of yeast and human α 1,2-mannosidases of the endoplasmic reticulum that convert Man9GlcNAc2 to Man8GlcNAc2 isomer B. The second subgroup comprising of mammalian Golgi α1,2-Mannosidases IA, IB, and IC cleave Man9GlcNAc2 to Man5GlcNAc2 via Man8GlcNAc2 isomer A and C. The third subgroup of yeast and mammalian proteins do not show any activity on Man9GlcNAc2. Proteins from subgroup 1 and 3 have been implicated in ER quality control and in proteasomal degradation of misfolded glycoproteins. The Golgi mannosidases from the second subgroup may play a role in the ERAD (endoplasmic reticulum-associated degradation) of defective glycoproteins. The three subgroups have been found to pariticipate in the maturation of Asn-linked glycoproteins in the endoplasmic reticulum (ER) and Golgi complex.

Physical form

Solution in 0.01 M phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Yuri D Lobsanov et al.
The Journal of biological chemistry, 277(7), 5620-5630 (2001-11-21)
Class I alpha1,2-mannosidases (glycosylhydrolase family 47) are key enzymes in the maturation of N-glycans. This protein family includes two distinct enzymatically active subgroups. Subgroup 1 includes the yeast and human endoplasmic reticulum (ER) alpha1,2-mannosidases that primarily trim Man(9)GlcNAc(2) to Man(8)GlcNAc(2)
Identification of cholinergic chemosensory cells in mouse tracheal and laryngeal glandular ducts
Krasteva-Christ G, et al.
International Immunopharmacology, 29(1), 158-165 (2015)
Shijiao Huang et al.
Cell reports, 41(8), 111679-111679 (2022-11-24)
N-glycans are processed mainly in the Golgi, and a well-organized Golgi structure is required for accurate glycosylation. However, during mitosis the Golgi undergoes severe fragmentation. The resulting trafficking block leads to an extended exposure of cargo molecules to Golgi enzymes.
O-GlcNAcylation mediates metastasis of cholangiocarcinoma through FOXO3 and MAN1A1
Phoomak C, et al.
Oncogene, 37(42), 5648-5648 (2018)
Wolfram Tempel et al.
The Journal of biological chemistry, 279(28), 29774-29786 (2004-04-23)
Three subfamilies of mammalian Class 1 processing alpha1,2-mannosidases (family 47 glycosidases) play critical roles in the maturation of Asn-linked glycoproteins in the endoplasmic reticulum (ER) and Golgi complex as well as influencing the timing and recognition for disposal of terminally

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