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09-275

Sigma-Aldrich

Anti-phospho-JAK2 (Tyr1007/1008) Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Tyrosine-protein kinase JAK2, Janus kinase 2, JAK-2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

pig, mouse

species reactivity (predicted by homology)

rat (based on 100% sequence homology), human (based on 100% sequence homology), chicken (based on 100% sequence homology), Xenopus (based on 100% sequence homology), zebrafish (based on 100% sequence homology), porcine (based on 100% sequence homology)

technique(s)

western blot: suitable

NCBI accession no.

NP_004963

UniProt accession no.

O60674

shipped in

wet ice

target post-translational modification

phosphorylation (pTyr1007/pTyr1008)

Gene Information

chicken ... Jak2(374199)
frog ... Jak2(100036599)
human ... JAK2(3717)
mouse ... Jak2(16452)
pig ... Jak2(397201)
rat ... Jak2(24514)

General description

Janus kinase 2 (JAK2) is a ubiquitous non-receptor tyrosine kinase that interacts with multiple cell-surface receptors and non-receptor proteins to affect a range of cellular processes including immunity, cell growth, development, and differentiation. Among JAK2’s receptor partners are the prolactin, growth hormone, thrombopoitin, and INF (α and β) receptors. JAK2 plays an important role in connecting the signaling pathways of these receptors to the activation of target genes. Upon ligand binding these receptors activate JAK2 inducing JAK2 autophosphorylation; This activated JAK2 in turn phosphorylates the cytoplasmic domains of these receptors providing a binding site for STAT transcription factors which are also phosphorylated by JAK2 leading to STAT protein dimerization and translocation to the nucleus to effect gene transcription. Aberrations in JAK2 expression and function may contribute to myelofibrosis and myelogenous leukemia.

Specificity

This antibody recognizes JAK2 phosphorylated at (Tyr1007/Tyr1008).

Immunogen

Epitope: Phosphorylated (Tyr1007/Tyr1008)
KLH-conjugated linear peptide corresponding to human JAK2 phosphorylated at (Tyr1007/Tyr1008).

Application

Anti-phospho-JAK2 (Tyr1007/1008) Antibody is an antibody against phospho-JAK2 (Tyr1007/1008) for use in WB.
Research Category
Signaling

Quality

Evaluated by Western Blot in LPS/IFN gamma untreated and treated RAW264.7 cell lysates.

Western Blot Analysis: A 1:5,000 dilution of this antibody detected JAK2 on 10 µg of LPS/IFN gamma untreated and treated RAW264.7 cell lysates.

Target description

~130 kDa observed. Uncharacterized bands appear at ~48, 55, 65, and 100 kDa in some lysates.

Linkage

Replaces: 04-1098

Physical form

Affinity purified
Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl, and 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
LPS/IFN gamma untreated and treated RAW264.7 cell lysates

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Céline S Nicolas et al.
Neuron, 73(2), 374-390 (2012-01-31)
The Janus kinase (JAK)/signal transducer and activator of transcription (STAT) pathway is involved in many cellular processes, including cell growth and differentiation, immune functions and cancer. It is activated by various cytokines, growth factors, and protein tyrosine kinases (PTKs) and
Wei Tong et al.
The Journal of biological chemistry, 281(50), 38930-38940 (2006-10-21)
Janus tyrosine kinase 2 (JAK2) is essential for signaling by the thrombopoietin (TpoR) and erythropoietin (EpoR) receptors. In the absence of JAK2 most EpoR molecules are retained in the endoplasmic reticulum in an Endo H-sensitive form. In contrast, we show
Rama S Kota et al.
The Journal of biological chemistry, 280(35), 31076-31084 (2005-07-05)
Lipoprotein lipase (LPL) is a key enzyme in the hydrolysis of triglyceride-rich lipoproteins. In vascular diseases, such as atherosclerosis, inflammation plays an important role in the pathogenesis of the disease. We examined the role of LPL in modulating tumor necrosis
15-Lipoxygenase-1-enhanced Src-Janus kinase 2-signal transducer and activator of transcription 3 stimulation and monocyte chemoattractant protein-1 expression require redox-sensitive activation of epidermal growth factor receptor in vascular wall remodeling.
Singh, NK; Wang, D; Kundumani-Sridharan, V; Van Quyen, D; Niu, J; Rao, GN
The Journal of Biological Chemistry null
Han-Chung Lee et al.
Scientific reports, 11(1), 3847-3847 (2021-02-17)
Ruxolitinib is the first janus kinase 1 (JAK1) and JAK2 inhibitor that was approved by the United States Food and Drug Administration (FDA) agency for the treatment of myeloproliferative neoplasms. The drug targets the JAK/STAT signalling pathway, which is critical
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