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column	Ascentis Express OH5 15 cm x 4.6 mm, 5 µm particles (50347-U)
column temp.	40 °C
mobile phase	[A] 0.005M Ammounium formate in Water (pH: 3.15 with Trifluoroacetic Acid) ), [B] Acetonitrile (30:70, A:B)
flow rate	1.0 mL/min
pressure \r\n \r\n	914 psi (63 bar)
sample	0.8 mg/mL water:acetonitrile (1:1)
injection	3 µL
detector	Agilent 380-ELSD

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Analysis Note	This USP application was generated by RTC on an Ascentis^® Express OH5 column.
Categories	Analytical Chromatography, Amino Acids
Featured Industry	Life Science and Biopharma
suitability	application for HPLC

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