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Column	SLB-35ms, 10 m x 0.25 mm I.D., 0.10 µm; made by cutting down a 30 m x 0.25 mm I.D., 0.10 µm column (29802-U)
Oven	75 °C (0.1 min), 15 °C/min to 380 °C (10 min)
Carrier gas	helium, 1.5 mL/min
Sample	coconut oil (organic, extra virgin), 0.02% (v/v) in chloroform
Injection	1 µL, on-column
Inj. temp.	track oven: 75 °C (0.1 min), 15 °C/min to 380 °C (10 min)
Detector	FID, 380 °C

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Analysis Note	Triglycerides are the main constituent of vegetable oil and animal fat, and make up most of the fats digested by humans. Most natural fats contain a complex mixture of individual triglycerides. Therefore, efficient capillary GC columns are required for ample resolution and subsequent identification. Triglycerides are large compounds, and require a relatively high final oven temperature for GC analysis. For the analysis of triglycerides in extra virgin coconut oil, a short, thin film version of SLB-35ms (cut-down from a longer column) run to a final oven temperature of 380 ºC was found to provide good resolution and reasonable elution times.
Categories	Analytical Chromatography, GC Applications, Glycerides
Featured Industry	Agriculture \nFood and Beverages
Legal Information	SLB is a registered trademark of Sigma-Aldrich Co. LLC
Suitability	Application for GC

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