TLC Analysis of Estradiol and Some of its Esters on HPTLC Silica Gel 60 RP-18 WF 254s with concentrating zone
Materials
부속물
제품 번호
설명
가격
Hamilton® TLC syringes
1702, volume 25 μL, needle size 22s ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1705, volume 50 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
Hamilton® TLC syringes
1710, volume 100 μL, needle size 22 ga, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate RP-18 wF 254s with concentraling zone (1.13124.0001)
eluent
[A] acetonitrile; [B] water; (75:25, A:B)
migration distance
6 cm
chamber
normal chamber without chamber saturation
sample
5 μL
detector
Dipped in MnCl2-sulfuric acid heated up to 110° C for 10 min (in situ evaluation with TLC/HPTLC scanner (Camag) UV 366 nm. )