HPLC Analysis of a Monoclonal Antibody on Zenix^®-C SEC-150 after Partial Proteolysis with IdeS Protease

Materials

07298

FabRICATOR^®

for cleaving 2 mg IgG, 2000 U/vial

CONDITIONS

column

Zenix-C SEC-150, 30 cm X 4.6 mm I.D., 3 μm particles (Z777083)

mobile phase

0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide (567329-U)

flow rate

0.35 mL/min

pressure

1450 psi (100 bar)

column temp.

ambient

detector

UV, 215 nm

injection

1 μL

sample

digested SO57 mAb, ~0.5 g/L

설명

분석 메모

Proteolysis of antibodies can be useful for simplifying further analysis. This application demonstrates separation of the two fragments, F(ab′)2 and scFc from the intact IgG, after partial digestion with IdeS protease.

법적 정보

Zenix is a registered trademark of Sepax Technologies

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HPLC Analysis of a Monoclonal Antibody on Zenix®-C SEC-150 after Partial Proteolysis with IdeS Protease