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  • Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature.

Control of actin polymerization via the coincidence of phosphoinositides and high membrane curvature.

The Journal of cell biology (2017-09-20)
Frederic Daste, Astrid Walrant, Mikkel R Holst, Jonathan R Gadsby, Julia Mason, Ji-Eun Lee, Daniel Brook, Marcel Mettlen, Elin Larsson, Steven F Lee, Richard Lundmark, Jennifer L Gallop
초록

The conditional use of actin during clathrin-mediated endocytosis in mammalian cells suggests that the cell controls whether and how actin is used. Using a combination of biochemical reconstitution and mammalian cell culture, we elucidate a mechanism by which the coincidence of PI(4,5)P

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Sigma-Aldrich
Latrunculin A, from sea sponge, ≥85% (HPLC), waxy solid
Sigma-Aldrich
Wortmannin, from Penicillium funiculosum, ≥98% (HPLC and TLC)
Sigma-Aldrich
MISSION® esiRNA, targeting human AP2M1