Plasticity of Human Meniscus Fibrochondrocytes: A Study on Effects of Mitotic Divisions and Oxygen Tension.

Plasticity of Human Meniscus Fibrochondrocytes: A Study on Effects of Mitotic Divisions and Oxygen Tension.

Scientific reports (2017-09-25)

Yan Liang, Enaam Idrees, Stephen H J Andrews, Kirollos Labib, Alexander Szojka, Melanie Kunze, Andrea D Burbank, Aillette Mulet-Sierra, Nadr M Jomha, Adetola B Adesida

PMID28939894

초록

Meniscus fibrochondrocytes (MFCs) may be the optimal cell source to repair non-healing meniscus injuries using tissue engineering strategies. In this study, we investigated the effects of mitotic divisions and oxygen tension on the plasticity of adult human MFCs. Our assessment techniques included gene expression, biochemical, histological, and immunofluorescence assays. MFCs were expanded in monolayer culture with combined growth factors TGFβ1 and FGF-2 (T1F2) under normoxia (21% O

MATERIALS

제품 번호

브랜드

제품 설명

H6254

Sigma-Aldrich

Hyaluronidase from sheep testes, Type V, lyophilized powder, ≥1,500 units/mg solid