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Merck
  • TIM29 is a subunit of the human carrier translocase required for protein transport.

TIM29 is a subunit of the human carrier translocase required for protein transport.

FEBS letters (2016-10-28)
Sylvie Callegari, Frank Richter, Katarzyna Chojnacka, Daniel C Jans, Isotta Lorenzi, David Pacheu-Grau, Stefan Jakobs, Christof Lenz, Henning Urlaub, Jan Dudek, Agnieszka Chacinska, Peter Rehling
초록

Hydrophobic inner mitochondrial membrane proteins with internal targeting signals, such as the metabolite carriers, use the carrier translocase (TIM22 complex) for transport into the inner membrane. Defects in this transport pathway have been associated with neurodegenerative disorders. While the TIM22 complex is well studied in baker's yeast, very little is known about the mammalian TIM22 complex. Using immunoprecipitation, we purified the human carrier translocase and identified a mitochondrial inner membrane protein TIM29 as a novel component, specific to metazoa. We show that TIM29 is a constituent of the 440 kDa TIM22 complex and interacts with oxidized TIM22. Our analyses demonstrate that TIM29 is required for the structural integrity of the TIM22 complex and for import of substrate proteins by the carrier translocase.

MATERIALS
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Sigma-Aldrich
MISSION® esiRNA, targeting human TIMM22
Sigma-Aldrich
GeneJuice® Transfection Reagent, Non-lipid based chemical transfection reagent optimized for maximum transfection efficiency, ease-of-use, and minimal cytotoxicity on a wide variety of mammalian cells.