콘텐츠로 건너뛰기
Merck
  • Eph-mediated tyrosine phosphorylation of citron kinase controls abscission.

Eph-mediated tyrosine phosphorylation of citron kinase controls abscission.

The Journal of cell biology (2016-08-24)
Thomas Jungas, Renaud T Perchey, Mohamad Fawal, Caroline Callot, Carine Froment, Odile Burlet-Schiltz, Arnaud Besson, Alice Davy
초록

Cytokinesis is the last step of cell division, culminating in the physical separation of daughter cells at the end of mitosis. Cytokinesis is a tightly regulated process that until recently was mostly viewed as a cell-autonomous event. Here, we investigated the role of Ephrin/Eph signaling, a well-known local cell-to-cell communication pathway, in cell division. We show that activation of Eph signaling in vitro leads to multinucleation and polyploidy, and we demonstrate that this is caused by alteration of the ultimate step of cytokinesis, abscission. Control of abscission requires Eph kinase activity, and Src and citron kinase (CitK) are downstream effectors in the Eph-induced signal transduction cascade. CitK is phosphorylated on tyrosines in neural progenitors in vivo, and Src kinase directly phosphorylates CitK. We have identified the specific tyrosine residues of CitK that are phosphorylated and show that tyrosine phosphorylation of CitK impairs cytokinesis. Finally, we show that, similar to CitK, Ephrin/Eph signaling controls neuronal ploidy in the developing neocortex. Our study indicates that CitK integrates intracellular and extracellular signals provided by the local environment to coordinate completion of cytokinesis.

MATERIALS
제품 번호
브랜드
제품 설명

Roche
Rapid DNA Ligation Kit
Sigma-Aldrich
Anti-NeuN Antibody, clone A60, clone A60, Chemicon®, from mouse