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In situ formation of H2O2 for P450 peroxygenases.

In situ formation of H2O2 for P450 peroxygenases.

Bioorganic & medicinal chemistry (2014-07-06)

Caroline E Paul, Ekaterina Churakova, Elmer Maurits, Marco Girhard, Vlada B Urlacher, Frank Hollmann

초록

An in situ H2O2 generation approach to promote P450 peroxygenases catalysis was developed through the use of the nicotinamide cofactor analogue 1-benzyl-1,4-dihydronicotinamide (BNAH) and flavin mononucleotide (FMN). Final productivity could be enhanced due to higher enzyme stability at low H2O2 concentrations. The H2O2 generation represented the rate-limiting step, however it could be easily controlled by varying both FMN and BNAH concentrations. Further characterization can result in an optimized ratio of FMN/BNAH/O2/biocatalyst enabling high reaction rates while minimizing H2O2-related inactivation of the enzyme.

MATERIALS

제품 번호

브랜드

제품 설명

Sigma-Aldrich

Dodecanoic acid, ≥99% (GC/titration)

Sigma-Aldrich

Dimethyl sulfoxide, ≥99.5% (GC), suitable for plant cell culture

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Dimethyl sulfoxide, meets EP testing specifications, meets USP testing specifications

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Dimethyl sulfoxide, PCR Reagent

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Sigma 7-9^®, ≥99% (titration), crystalline

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Dimethyl sulfoxide, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

Sigma-Aldrich

Dimethyl sulfoxide, BioUltra, for molecular biology, ≥99.5% (GC)

Supelco

Dimethyl sulfoxide, analytical standard

Supelco

Dodecanoic acid, analytical standard

Millipore

Ethyl acetate, suitable for HPLC, ≥99.8% (GC)

Supelco

Ethyl acetate, analytical standard

Sigma-Aldrich

Ethyl acetate, ≥99%, FCC, FG

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Chlorotrimethylsilane solution, 1.0 M in THF

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Ethyl acetate, natural, ≥99%, FCC, FG

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Dodecanoic acid, 98%

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Dimethyl sulfoxide, anhydrous, ≥99.9%

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Ethyl acetate, anhydrous, 99.8%

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Ethyl acetate, ReagentPlus^®, ≥99.8%

Sigma-Aldrich

8-Octanoyloxypyrene-1,3,6-trisulfonic acid trisodium salt, suitable for fluorescence, ≥90% (HPCE)

Supelco

Dimethyl sulfoxide, for inorganic trace analysis, ≥99.99995% (metals basis)

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Ethyl acetate, ACS reagent, ≥99.5%

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Trifluoroacetamide, 97%

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Ethyl acetate, puriss., meets analytical specification of Ph. Eur., BP, NF, ≥99.5% (GC)

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Ethyl acetate, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.5% (GC)

Sigma-Aldrich

Dimethyl sulfoxide, ReagentPlus^®, ≥99.5%

Sigma-Aldrich

Dimethyl sulfoxide, ACS reagent, ≥99.9%

Sigma-Aldrich

Ethyl acetate, ACS reagent, ≥99.5%

Sigma-Aldrich

Dimethyl sulfoxide, puriss. p.a., dried, ≤0.02% water

SAFC

Tromethamine

Dimethyl sulfoxide, European Pharmacopoeia (EP) Reference Standard