콘텐츠로 건너뛰기

The histidine kinase CusS senses silver ions through direct binding by its sensor domain.

The histidine kinase CusS senses silver ions through direct binding by its sensor domain.

Biochimica et biophysica acta (2014-06-21)

Swapna A Gudipaty, Megan M McEvoy

초록

The Cus system of Escherichia coli aids in protection of cells from high concentrations of Ag(I) and Cu(I). The histidine kinase CusS of the CusRS two-component system functions as a Ag(I)/Cu(I)-responsive sensor kinase and is essential for induction of the genes encoding the CusCFBA efflux pump. In this study, we have examined the molecular features of the sensor domain of CusS in order to understand how a metal-responsive histidine kinase senses specific metal ions. We find that the predicted periplasmic sensor domain of CusS directly interacts with Ag(I) ions and undergoes a conformational change upon metal binding. Metal binding also enhances the tendency of the domain to dimerize. These findings suggest a model for activation of the histidine kinase through metal binding events in the periplasmic sensor domain.

MATERIALS

제품 번호

브랜드

제품 설명

Copper - O.F.H.C., foil, 300x300mm, thickness, 0.5mm, half hard, flattened, 99.95+%

Copper - O.F.H.C., foil, 2m coil, thickness 0.20mm, hard, 99.95+%

Copper - O.F.H.C., foil, 5m coil, thickness 0.075mm, half hard, 99.95+%

Copper, wire reel, 100m, diameter 0.5mm, as drawn, 99.98+%

Copper, wire reel, 500m, diameter 0.04mm, annealed, 99.9%

Copper, wire reel, 1m, diameter 0.75mm, as drawn, 99.98+%

Copper, wire reel, 100m, diameter 0.5mm, annealed, silver plated, 99.9%

Copper - O.F.H.C., foil, 5m coil, thickness 0.125mm, hard, 99.95+%

Silver, foil, light tested, 100x100mm, thickness 0.02mm, 99.97%

Copper, wire reel, 100m, diameter 0.025mm, as drawn, 99.99+%

Silver, foil, 5m coil, thickness 0.0125mm, coil width 9mm, 99.95+%

Copper, wire reel, 100m, diameter 0.02mm, hard, 99.99+%

Copper, wire reel, 500m, diameter 0.05mm, annealed, 99.9%

Copper, wire reel, 1m, diameter 0.01mm, hard, 99.99%

Copper - O.F.H.C., foil, 2m coil, thickness 0.05mm, hard, 99.95+%

Copper, wire reel, 50m, diameter 0.02mm, hard, 99.99+%

Copper, wire reel, 25m, diameter 0.75mm, as drawn, 99.98+%

Copper, wire reel, 2000m, diameter 0.1mm, annealed, 99.9%

Copper, wire reel, 20m, diameter 1.0mm, as drawn, 99.98+%

Copper, wire reel, 100m, diameter 1.0mm, annealed, 99.9%

Copper, wire reel, 200m, diameter 0.5mm, annealed, silver plated, 99.9%

Copper, wire reel, 10m, diameter 1.0mm, annealed, 99.9%

Copper, wire reel, 50m, diameter 0.25mm, as drawn, 99.99+%

Copper, wire reel, 100m, diameter 0.075mm, annealed, 99.9%

Silver, foil, light tested, 50x50mm, thickness 0.025mm, as rolled, 99.99+%

Copper, wire reel, 1000m, diameter 0.05mm, annealed, 99.9%

Copper, wire reel, 50m, diameter 0.1mm, annealed, 99.9%

Silver, foil, not light tested, 100x100mm, thickness 0.006mm, 99.97%

Copper, tube, 500mm, outside diameter 1.75mm, inside diameter 0.33mm, wall thickness 0.71mm, as drawn, 99.9%

Copper, tube, 200mm, outside diameter 12.7mm, inside diameter 10.26mm, wall thickness 1.22mm, as drawn, 99.9%