Avanti lipid tools: connecting lipids, technology, and cell biology.

Avanti lipid tools: connecting lipids, technology, and cell biology.

Biochimica et biophysica acta (2014-06-24)

Kacee H Sims, Ewan M Tytler, John Tipton, Kasey L Hill, Stephen W Burgess, Walter A Shaw

초록

Lipid research is challenging owing to the complexity and diversity of the lipidome. Here we review a set of experimental tools developed for the seasoned lipid researcher, as well as, those who are new to the field of lipid research. Novel tools for probing protein-lipid interactions, applications for lipid binding antibodies, enhanced systems for the cellular delivery of lipids, improved visualization of lipid membranes using gold-labeled lipids, and advances in mass spectrometric analysis techniques will be discussed. Because lipid mediators are known to participate in a host of signal transduction and trafficking pathways within the cell, a comprehensive lipid toolbox that aids the science of lipidomics research is essential to better understand the molecular mechanisms of interactions between cellular components. This article is part of a Special Issue entitled Tools to study lipid functions.

MATERIALS

제품 번호

브랜드

제품 설명

326542

Sigma-Aldrich

Gold, beads, 1-6 mm, 99.999% trace metals basis

326585

Sigma-Aldrich

Gold, powder, <10 μm, ≥99.9% trace metals basis

349305

Sigma-Aldrich

Gold, wire, diam. 1.0 mm, 99.997% trace metals basis

326526

Sigma-Aldrich

Gold, wire, diam. 0.127 mm, 99.99% trace metals basis

326534

Sigma-Aldrich

Gold, wire, diam. 0.25 mm, ≥99.9% trace metals basis

265772

Sigma-Aldrich

Gold, powder, <45 μm, 99.99% trace metals basis

265837

Sigma-Aldrich

Gold, rod, diam. 3.0 mm, 99.99% trace metals basis

349283

Sigma-Aldrich

Gold, wire, diam. 0.1 mm, 99.99% trace metals basis

265829

Sigma-Aldrich

Gold, foil, thickness 0.5 mm, 99.99% trace metals basis

349240

Sigma-Aldrich

Gold, foil, thickness 0.25 mm, ≥99.9% trace metals basis

349275

Sigma-Aldrich

Gold, foil, thickness 0.05 mm, 99.99% trace metals basis

373168

Sigma-Aldrich

Gold, evaporation slug, diam. × L 0.6 cm × 0.6 cm, 99.99% trace metals basis

310980

Sigma-Aldrich

Gold, wire, diam. 0.5 mm, 99.99% trace metals basis

GF91804271

Gold, insulated wire, 2m, conductor diameter 0.075mm, insulation thickness 0.012mm, PTFE (polytetrafluoroethylene) insulation, 99.99%

GF46990974

Gold, microfoil, disks, 10mm, thinness 0.05μm, specific density 101.3μg/cm², permanent mylar 3.5μm support, 99.99+%

GF39029968

Gold, rod, 100mm, diameter 2.0mm, as drawn, 99.95%

GF23782350

Gold, microfoil, disks, 10mm, thinness 0.1μm, specific density 204.1μg/cm², permanent mylar 3.5μm support, 99.99+%

GF92960286

Gold, tube, 50mm, outside diameter 8mm, inside diameter 7.8mm, wall thickness 0.1mm, as drawn, 99.95%

GF04839767

Gold, tube, 100mm, outside diameter 1.60mm, inside diameter 0.6mm, wall thickness 0.5mm, as drawn, 99.95%

GF73794741

Gold, tube, 200mm, outside diameter 2.0mm, inside diameter 1.8mm, wall thickness 0.1mm, as drawn, 99.95%

GF98812097

Gold, rod, 6mm, diameter 6.0mm, as drawn, 99.95%

GF03557336

Gold, rod, 10mm, diameter 6.0mm, as drawn, 99.95%

GF05586969

Gold, tube, 100mm, outside diameter 3.0mm, inside diameter 2.8mm, wall thickness 0.10mm, as drawn, 99.95%

GF26034320

Gold, tube, 100mm, outside diameter 10.0mm, inside diameter 9.7mm, wall thickness 0.15mm, as drawn, 99.95%

GF37996789

Gold, tube, 200mm, outside diameter 2.0mm, inside diameter 1.7mm, wall thickness 0.15mm, as drawn, 99.95%

GF75346678

Gold, insulated wire, 0.5m, conductor diameter 0.075mm, insulation thickness 0.012mm, PTFE (polytetrafluoroethylene) insulation, 99.99%

GF33293729

Gold, insulated wire, 0.1m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%

GF38598980

Gold, microfoil, disks, 10mm, thinness 0.5μm, specific density 966μg/cm², permanent mylar 3.5μm support, 99.99+%

GF88214542

Gold, insulated wire, 1m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%

GF92307922

Gold, insulated wire, 5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%