Surface characterization and osteoblast response to a functionally graded hydroxyapatite/fluoro-hydroxyapatite/titanium oxide coating on titanium surface by sol-gel method.

Surface characterization and osteoblast response to a functionally graded hydroxyapatite/fluoro-hydroxyapatite/titanium oxide coating on titanium surface by sol-gel method.

Cell proliferation (2014-04-18)

G He, B Guo, H Wang, C Liang, L Ye, Y Lin, X Cai

초록

To improve efficacy of current titanium and its alloys, in bioactivity and speed of osseointegration, of orthopaedic implants. A novel triple-layered functional graded coating, consisting of a porous hydroxyapatite (HA) outermost layer, fluoro-HA (FHA) intermediate layer and titanium oxide (TiO2 ) innermost layer, was created on a titanium substrate by a multistep sol-gel method. X-ray diffraction analysis showed TiO2 anatase and apatite crystallization in the coating. Morphological analysis performed by scanning electron microscopy showed excellent bonding between coating and substrate, with a thickness of ~2 μm. Scratch testing found favourable adhesion strength of the composite coating. In addition, optical microscope images suggested good biocompatibility. Considering thet in vitro cell response, osteoblasts on the coating exhibited higher cell proliferation and ALP activity compared to pure titanium and HA coating, and demonstrated excellent coating bioactivity. Current results indicated that the novel TiO2 /FHA/HA coating has promising clinical applications in orthopaedic and dental implantation.

MATERIALS

제품 번호

브랜드

제품 설명

460400

Sigma-Aldrich

Titanium, wire, diam. 0.25 mm, 99.7% trace metals basis

204757

Sigma-Aldrich

Titanium(IV) oxide, rutile, ≥99.98% trace metals basis

266035

Sigma-Aldrich

Titanium, wire, diam. 1.0 mm, 99.99% trace metals basis

305812

Sigma-Aldrich

Titanium, 5-10 mm, ≥99.99% trace metals basis (purity exclusive of Na and K content)

267902

Sigma-Aldrich

Titanium, wire, diam. 0.81 mm, 99.7% trace metals basis

369489

Sigma-Aldrich

Titanium, foil, thickness 2.0 mm, 99.7% trace metals basis

IRMM531C

Titanium, IRMM^®, certified reference material, 0.5 mm wire

IRMM531B

Titanium, IRMM^®, certified reference material, 0.5 mm foil

04238

Sigma-Aldrich

Hydroxyapatite, puriss., meets analytical specification of Ph. Eur., BP, FCC, E341, ≥90% (calculated on glowed substance)

21223

Sigma-Aldrich

Hydroxyapatite, purum p.a., ≥90% (as Ca₃(PO₄)₂, KT)

204730

Sigma-Aldrich

Titanium(IV) oxide, rutile, 99.995% trace metals basis

348848

Sigma-Aldrich

Titanium, foil, thickness 0.025 mm, 99.98% trace metals basis

637262

Sigma-Aldrich

Titanium(IV) oxide, rutile, nanopowder, <100 nm particle size, 99.5% trace metals basis

224227

Sigma-Aldrich

Titanium(IV) oxide, rutile, powder, <5 μm, ≥99.9% trace metals basis

700347

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanoparticles, <150 nm particle size (volume distribution, DLS), dispersion, 40 wt. % in H₂O, 99.5% trace metals basis

634662

Sigma-Aldrich

Titanium(IV) oxide, mixture of rutile and anatase, nanopowder, <100 nm particle size (BET), 99.5% trace metals basis

348791

Sigma-Aldrich

Titanium, foil, thickness 0.127 mm, 99.7% trace metals basis

GF51463357

Titanium, tube, 200mm, outside diameter 10.3mm, inside diameter 8.7mm, wall thickness 0.8mm, annealed, 99.6+%

GF41437830

Titanium, tube, 100mm, outside diameter 1.05mm, inside diameter 0.75mm, wall thickness 0.15mm, annealed, 99.6+%

GF72212813

Titanium, tube, 200mm, outside diameter 25.4mm, inside diameter 23.62mm, wall thickness 0.89mm, annealed, 99.6+%

GF13915447

Titanium, tube, 100mm, outside diameter 2.03mm, inside diameter 1.55mm, wall thickness 0.24mm, annealed, 99.6+%

GF05438066

Titanium, tube, 1000mm, outside diameter 1.6mm, inside diameter 1.2mm, wall thickness 0.2mm, hard, 99.6+%

GF65515024

Titanium, tube, 1000mm, outside diameter 12.7mm, inside diameter 10.9mm, wall thickness 0.9mm, annealed, 99.6+%

GF35061083

Titanium, tube, 200mm, outside diameter 6.35mm, inside diameter 5.53mm, wall thickness 0.41mm, annealed, 99.6+%

GF85496664

Titanium, tube, 1000mm, outside diameter 9.5mm, inside diameter 8.2mm, wall thickness 0.65mm, annealed, 99.6+%

GF90963928

Titanium, rod, 25mm, diameter 50mm, 99.99+%

GF48167590

Titanium, tube, 100mm, outside diameter 6.1mm, inside diameter 5.1mm, wall thickness 0.5mm, annealed, 99.6+%

GF11909932

Titanium, tube, 100mm, outside diameter 4.25mm, inside diameter 3.75mm, wall thickness 0.25mm, annealed, 99.6+%

GF43519945

Titanium, wire reel, 2m, diameter 1.0mm, as drawn, 99.99+%

GF00761217

Titanium, wire reel, 200m, diameter 0.25mm, as drawn, 99.6+%