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  • Application of graphene-based solid-phase extraction for ultra-fast determination of malachite green and its metabolite in fish tissues.

Application of graphene-based solid-phase extraction for ultra-fast determination of malachite green and its metabolite in fish tissues.

Food chemistry (2013-06-26)
Linyao Chen, Yanbin Lu, Shiyao Li, Xianjiang Lin, Zhongming Xu, Zhiyuan Dai
초록

An ultra-fast analytical protocol using graphene-based solid-phase extraction coupled with ultra-performance liquid chromatography-tandem mass spectrometry for the rapid determination of malachite green and its metabolite, leucomalachite green in fish tissues has been developed. In the present work, graphene was synthesized and evaluated as novel solid-phase extraction sorbents for the analytes enrichment and clean-up. The target analytes were separated on an ultra-pressure BEH C8 column and quantified by a triple-quadrupole linear ion trap mass spectrometer in multiple-reaction monitoring mode. The proposed analytical procedures were carefully optimized and validated. The matrix-matched calibration curves were performed at six concentration levels and good linear relationship (R(2)>0.9990) was observed within the range of 0.25-50μgkg(-1). The proposed method has been successfully applied to the analysis of MG and LMG in several fish samples, indicating that graphene was an efficient SPE sorbent for the enrichment of trace residues in food analysis.

MATERIALS
제품 번호
브랜드
제품 설명

Supelco
Malachite Green oxalate salt, VETRANAL®, analytical standard
Sigma-Aldrich
Malachite Green oxalate salt, for microscopy, crystalline, S. No.: 754
Sigma-Aldrich
Malachite Green oxalate salt, Technical grade
Sigma-Aldrich
Schaeffer and Fulton Spore Stain Solution A, for microscopy
Sigma-Aldrich
Malachite Green oxalate salt, certified by the Biological Stain Commission
Supelco
Malachite Green chloride, analytical standard
Sigma-Aldrich
Malachite Green solution, for microscopy