Experimental allergic encephalomyelitis: simple method for producing the localized form.

In the past, the lesions of experimental allergic encephalomyelitis (EAE) have been induced to localize around brain tissue damaged by anoxia or direct physical or chemical attack. The procedure for producing the requisite antecedent brain injury has been simplified by use of a single subcutaneous injection of a neurotoxic chemical, thereby eliminating the need for surgery. The EAE lesions are concentrated in a very small area and can be obtained in one day after the passive transfer of a relatively small number of lymph node cells from immunized donors.