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Merck
  • Expression of beta-galactosidase from a hybrid promoter:operator element in Escherichia coli.

Expression of beta-galactosidase from a hybrid promoter:operator element in Escherichia coli.

FEMS microbiology letters (1993-01-15)
K E Dixon, D F Fix
초록

A hybrid trpPO:lacO regulatory sequence was cloned upstream of a promoterless lacZ gene and recombined onto a lambda bacteriophage. Escherichia coli lysogens representing the four possible phenotypes for lacI and trpR were constructed and the synthesis of beta-galactosidase was assayed under various growth conditions. The results illustrated that both control elements could be efficiently and independently regulated by the addition or omission of appropriate accessory molecules.

MATERIALS
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제품 설명

Sigma-Aldrich
trans-3-Indoleacrylic acid, 98%
Sigma-Aldrich
3-Indoleacrylic acid, powder
Sigma-Aldrich
3-Indoleacrylic acid, ≥98% (HPLC), BioReagent