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Merck

Enhanced ethanol production from wheat straw by integrated storage and pre-treatment (ISP).

Enzyme and microbial technology (2013-01-01)
Volkmar Passoth, Muhammad Rizwan Tabassum, Harikrishnan A S Nair, Matilda Olstorpe, Ievgeniia Tiukova, Jerry Stรฅhlberg
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Integrated storage and pre-treatment (ISP) combines biopreservation of moist material under airtight conditions and pre-treatment. Moist wheat straw was inoculated with the biocontrol yeast Wickerhamomyces anomalus, the xylan degrading yeast Scheffersomyces stipitis or a co-culture of both. The samples and non-inoculated controls were stored at 4 or 15 ยฐC. The non-inoculated controls were heavily contaminated with moulds, in contrast to the samples inoculated with W. anomalus or S. stipitis. These two yeasts were able to grow on wheat straw as sole source of nutrients. When ethanol was produced from moist wheat straw stored for four weeks at 4 ยฐC with S. stipitis, an up to 40% enhanced yield (final yield 0.15 g ethanol per g straw dry weight) was obtained compared to a dry sample (0.107 g/g). In all other moist samples, stored for four weeks at 4 ยฐC or 15 ยฐC, 6-35% higher yields were obtained. Thus, energy efficient bio-preservation can improve the pre-treatment efficiency for lignocellulose biomass, which is a critical bottleneck in its conversion to biofuels.

MATERIALS
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Sigma-Aldrich
Sulfuric acid, puriss. p.a., 95-97% (T)
Supelco
Sulfuric acid, for the determination of nitrogen, ≥97.0%
Sigma-Aldrich
Sulfuric acid, 99.999%
Sigma-Aldrich
Sulfuric acid, ACS reagent, 95.0-98.0%
Sigma-Aldrich
Sulfuric acid solution, puriss. p.a., ≥25% (T)
Sigma-Aldrich
Sulfuric acid, puriss. p.a., for determination of Hg, ACS reagent, reag. ISO, reag. Ph. Eur., 95.0-97.0%
Sigma-Aldrich
Sulfuric acid, puriss., meets analytical specification of Ph. Eur., BP, 95-97%
Supelco
Sulfuric acid concentrate, 0.1 M H2SO4 in water (0.2N), eluent concentrate for IC