Laser-induced fluorescence detection of 9-fluorenylmethyl chloroformate derivatized amino acids in capillary electrophoresis.

Laser-induced fluorescence (LIF) was applied to the detection of 9-fluorenylmethyl chloroformate (FMOC-Cl) derivatized amino acids separated by capillary electrophoresis. Fluorescence excitation was provided by a pulsed, KrF laser operating at 248 nm. A limit of detection of 5 x 10(-10) M was obtained for FMOC-alanine (S/N = 2). Separation of FMOC-derivatized proline, hydroxyproline, and sarcosine was achieved with a 20 mM borate buffer (pH 9.2), and the separation of FMOC-derivatized amino acid standard mixture was obtained using a 20 mM borate buffer (pH 9.2) containing 25 mM sodium dodecyl sulfate.