콘텐츠로 건너뛰기
Merck
  • Sulfite species enhance carbon monoxide release from CO-releasing molecules: implications for the deoxymyoglobin assay of activity.

Sulfite species enhance carbon monoxide release from CO-releasing molecules: implications for the deoxymyoglobin assay of activity.

Analytical biochemistry (2012-05-09)
Samantha McLean, Brian E Mann, Robert K Poole
초록

Carbon monoxide-releasing molecules (CO-RMs) emulate the beneficial (e.g., anti-inflammatory) effects of CO in biology. CO release from CO-RMs is routinely determined in the presence of reduced deoxy-myoglobin by measuring the formation of carboxy-myoglobin (Mb-CO). Previous studies have highlighted discrepancies between the apparent CO release rates of some CO-RMs established using this assay versus other experimental data where a slower or more complex mechanism of release is suggested. It has been hypothesized that some CO-RMs require a CO acceptor, believed to be reduced myoglobin in Mb-CO assays, in order to facilitate the release of CO. Here, we show, for the first time, that CO is not liberated from the ruthenium (Ru)-based [Ru(CO)(3)Cl(2)](2) (CORM-2) and [Ru(CO)(3)Cl(glycinate)] (CORM-3) at an appreciable rate in the presence of reduced myoglobin alone. Rather, we confirm that it is the reducing agent sodium dithionite that facilitates release of CO from these CO-RMs. Other sulfite compounds, namely sodium sulfite and potassium metabisulfite, also promote the liberation of CO from CORM-3. We describe an alternative oxy-hemoglobin assay that eliminates dithionite and suggest that the efficacy of CO-RMs results from intracellular interactions with anions that facilitate CO delivery to therapeutic targets.

MATERIALS
제품 번호
브랜드
제품 설명

Sigma-Aldrich
Potassium disulfite, BioUltra, ≥97.0% (RT)
Sigma-Aldrich
Sodium sulfite, BioUltra, anhydrous, ≥98% (RT)
Sigma-Aldrich
Sodium sulfite, BioXtra, ≥98%
Sigma-Aldrich
Potassium disulfite, puriss., meets analytical specification of Ph.Eur., BP, NF, FCC, E224, 95.0-101.0% (iodometric), powder, 51.8-57.6% sulfur dioxide
Sigma-Aldrich
Sodium sulfite, ≥98%
Sigma-Aldrich
Potassium disulfite, ≥98%
Sigma-Aldrich
Sodium sulfite, puriss. p.a., reag. Ph. Eur., anhydrous, 98-100.0% (iodometric)
Sigma-Aldrich
Sodium sulfite, ACS reagent, ≥98.0%
Sigma-Aldrich
Sodium sulfite, puriss., meets analytical specification of Ph. Eur., BP, NF, anhydrous, 95.0-100.0% (iodometric)
Sigma-Aldrich
Sodium sulfite, anhydrous, Redi-Dri, reagent grade, ≥98%