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Merck

End-stage kidney disease: a never healing wound leading to another never healing wound, renal cancer.

Journal of nephrology (2023-07-13)
Janos Docs, Gyula Kovacs, Lehel Peterfi
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End-stage kidney disease and acquired cystic kidney disease are the final stages of chronic kidney disease, leading to loss of kidney function and frequent development of tumours. It has been suggested that an inflammatory microenvironment may be responsible for the progressive kidney remodelling and cancer development. Our aim was to analyse gene expression suggested to be involved in the remodelling of kidneys in end-stage kidney disease, and in the development of preneoplastic lesions and tumours. Immunohistochemistry was employed to assess the cellular localisation of differentย genes involved in these pathwaysย on representative tissue sections. Cellular (ฮฑSMA positive naรฏve activated fibroblasts, endothelial cells, macrophages) and non-cellular components (cytokines IL6, TGFฮฒ, IL1ฮฒ, CSF2, fibronectin, laminin, and matrix modifier proteases MMP9 and MMP12) of the inflammatory microenvironment were expressed in the kidneys of patients with end-stage kidney disease. IL6 and FN1 expressing naรฏve activated fibroblasts and recruited inflammatory cells were the most abundant cellular components of the inflammatory microenvironment. The progressive inflammatory and fibrotic processes in end-stage kidney disease have features recalling those ofย  a never healing wound and may explain the frequent development of kidney cancer.

MATERIALS
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Sigma-Aldrich
Anti-TGF ฮฒ1 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-LAMB3 antibody produced in rabbit, Prestige Antibodiesยฎ Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-MMP9 antibody produced in rabbit, IgG fraction of antiserum
Sigma-Aldrich
Anti-LAMA3 antibody produced in rabbit, Prestige Antibodiesยฎ Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution