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Merck

Establishment of bovine trophoblast stem cells.

Cell reports (2023-05-06)
Yinjuan Wang, Hao Ming, Leqian Yu, Jie Li, Linkai Zhu, Hai-Xi Sun, Carlos A Pinzon-Arteaga, Jun Wu, Zongliang Jiang
초록

Here, we report that a chemical cocktail (LCDM: leukemia inhibitory factor [LIF], CHIR99021, dimethinedene maleate [DiM], minocycline hydrochloride), previously developed for extended pluripotent stem cells (EPSCs) in mice and humans, enables de novo derivation and long-term culture of bovine trophoblast stem cells (TSCs). Bovine TSCs retain developmental potency to differentiate into mature trophoblast cells and exhibit transcriptomic and epigenetic (chromatin accessibility and DNA methylome) features characteristic of trophectoderm cells from early bovine embryos. The bovine TSCs established in this study will provide a model to study bovine placentation and early pregnancy failure.

MATERIALS
제품 번호
브랜드
제품 설명

Sigma-Aldrich
Anti-COX2/PTGS2 antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
CHIR99021, ≥98% (HPLC)
Sigma-Aldrich
Forskolin, For use in molecular biology applications