- Rat Embryonic Stem Cell Transgenesis.
Rat Embryonic Stem Cell Transgenesis.
Methods in molecular biology (Clifton, N.J.) (2023-03-31)
Elizabeth C Bryda, Hongsheng Men, Barbara J Stone
PMID36995677
초록
The availability of reliable germline competent rat embryonic stem cell (ESC) lines that can be genetically manipulated provides an important tool for generating new rat models. Here we describe the process for culturing rat ESCs, microinjecting the ESCs into rat blastocysts, and transferring the embryos to surrogate dams by either surgical or non-surgical embryo transfer techniques to produce chimeric animals with the potential to pass on the genetic modification to their offspring.
MATERIALS
제품 번호
브랜드
제품 설명
Sigma-Aldrich
apo-Transferrin human, powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)
Eppendorf® TransferTip (ES), sterile, set of 25 ea