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Merck

Rat Embryonic Stem Cell Transgenesis.

Methods in molecular biology (Clifton, N.J.) (2023-03-31)
Elizabeth C Bryda, Hongsheng Men, Barbara J Stone
초록

The availability of reliable germline competent rat embryonic stem cell (ESC) lines that can be genetically manipulated provides an important tool for generating new rat models. Here we describe the process for culturing rat ESCs, microinjecting the ESCs into rat blastocysts, and transferring the embryos to surrogate dams by either surgical or non-surgical embryo transfer techniques to produce chimeric animals with the potential to pass on the genetic modification to their offspring.

MATERIALS
제품 번호
브랜드
제품 설명

Sigma-Aldrich
apo-Transferrin human, powder, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)
Sigma-Aldrich
Sodium selenite, BioReagent, suitable for cell culture, ≥98%
Eppendorf® TransferTip (ES), sterile, set of 25 ea
Sigma-Aldrich
Accutase® solution, sterile-filtered, suitable for cell culture