- Production and characterization of adeno-associated viral vectors.
Production and characterization of adeno-associated viral vectors.
Nature protocols (2007-04-05)
Joshua C Grieger, Vivian W Choi, R Jude Samulski
PMID17406430
초록
The adeno-associated virus (AAV) is one of the most promising viral vectors for human gene therapy. As with any potential therapeutic system, a thorough understanding of it at the in vitro and in vivo levels is required. Over the years, numerous methods have been developed to better characterize AAV vectors. These methods have paved the way to a better understanding of the vector and, ultimately, its use in clinical applications. This review provides an up-to-date, detailed description of essential methods such as production, purification and titering and their application to characterize current AAV vectors for preclinical and clinical use.
MATERIALS
제품 번호
브랜드
제품 설명
Sigma-Aldrich
Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
Sigma-Aldrich
Proteinase K from Tritirachium album, buffered aqueous glycerol solution, for molecular biology, ≥800 units/mL
Roche
DIG DNA Labeling Kit, sufficient for 40 labeling reactions, kit of 1 (7 components), suitable for hybridization