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Merck

Disruption of autophagy by increased 5-HT alters gut microbiota and enhances susceptibility to experimental colitis and Crohn's disease.

Science advances (2021-11-06)
Sabah Haq, Huaqing Wang, Jensine Grondin, Suhrid Banskota, John K Marshall, Irfan I Khan, Usha Chauhan, Francine Cote, Yun Han Kwon, Dana Philpott, John H Brumell, Michael Surette, Gregory R Steinberg, Waliul I Khan
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[Figure: see text].

MATERIALS
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Sigma-Aldrich
5-Hydroxy-L-tryptophan, powder
Sigma-Aldrich
Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
Supelco
Rapamycin, VETRANALยฎ, analytical standard
Sigma-Aldrich
Serotonin hydrochloride, powder
Sigma-Aldrich
Lectin from Phaseolus vulgaris (red kidney bean), Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture