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  • Importance of Lignin Coniferaldehyde Residues for Plant Properties and Sustainable Uses.

Importance of Lignin Coniferaldehyde Residues for Plant Properties and Sustainable Uses.

ChemSusChem (2020-07-22)
Masanobu Yamamoto, Leonard Blaschek, Elena Subbotina, Shinya Kajita, Edouard Pesquet
초록

Increases in coniferaldehyde content, a minor lignin residue, significantly improves the sustainable use of plant biomass for feed, pulping, and biorefinery without affecting plant growth and yields. Herein, different analytical methods are compared and validated to distinguish coniferaldehyde from other lignin residues. It is shown that specific genetic pathways regulate amount, linkage, and position of coniferaldehyde within the lignin polymer for each cell type. This specific cellular regulation offers new possibilities for designing plant lignin for novel and targeted industrial uses.

MATERIALS
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Sigma-Aldrich
Trizma® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline
Sigma-Aldrich
Peroxidase from horseradish, Type VI, essentially salt-free, lyophilized powder, ≥250 units/mg solid (using pyrogallol)
Sigma-Aldrich
4-Hydroxy-3-methoxycinnamaldehyde, 98%
Sigma-Aldrich
Sinapyl alcohol, technical grade, 80%
Supelco
N,O-Bis(trimethylsilyl)trifluoroacetamide, for GC derivatization, LiChropur, ≥99.0%
Sigma-Aldrich
Pyridine, anhydrous, 99.8%
Sigma-Aldrich
Coniferyl alcohol, 98%
Sigma-Aldrich
Boron trifluoride diethyl etherate, purified by redistillation, ≥46.5% BF3 basis
Sigma-Aldrich
Lithium dodecyl sulfate, ≥98.5% (GC)
Sigma-Aldrich
Phloroglucinol, Used to detect the presence of wood fiber.
Sigma-Aldrich
Trizma® acetate, ≥99.0% (titration)