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Merck

Genetic manipulation of Leptospira biflexa.

Current protocols in microbiology (2008-09-05)
Hélène Louvel, Mathieu Picardeau
초록

The genus Leptospira belongs to the order Spirochaetales and is composed of both saprophytic and pathogenic members, such as Leptospira biflexa and L. interrogans, respectively. A major factor contributing to our ignorance of spirochetal biology has been the lack of methods available for genetic analysis of these organisms. In recent years, an E. coli-L. biflexa shuttle vector has been constructed and a system for targeted mutagenesis and random transposon mutagenesis of the saprophyte L. biflexa has been developed. These studies enable the use of L. biflexa as a model bacterium among spirochetes.

MATERIALS
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브랜드
제품 설명

Sigma-Aldrich
Spectinomycin ready made solution, 100 mg/mL in DMSO/H2O, 1:1, Cell culture tested