G005601

HPLC Analysis of Levothyroxine and Liothyronine on Ascentis^® Express ES-Cyano

application for HPLC

속성

• 기술: HPLC: suitable
• 검사 파라미터: column: Ascentis Express ES-Cyano, 10 cm x 2.1 mm I.D., 2.7 μm particles (53491-U); mobile phase: [A] water with 0.05% phosphoric acid; [B] acetonitrile with 0.05% phophoric acid; (60:40, A:B); flow rate: 1.5 mL/min; pressure: 3270 psi (225 bar); column temp.: 30 °C; detector: UV, 225 nm; injection: 5 μL; sample: 20 μg/mL in mobile phase
• 적합성: application for HPLC
• 응용 분야: clinical

설명

분석 메모

This application demonstrates the suitabilty of Ascentis Express ES-Cyano for the analysis of Levothyroxine according to USP Monograph 3301 with Liothyronine as a reference standard.

법적 정보

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

분석물

3,3′,5-Triiodo-L-thyronine sodium salt, powder, BioReagent, suitable for cell culture
Levothyroxine sodium hydrate, PESTANAL^®, analytical standard