추천 제품
생물학적 소스
mouse
재조합
expressed in E. coli
분석
≥98% (HPLC)
≥98% (SDS-PAGE)
형태
lyophilized
효능
<1.0 ng/mL ED50
분자량
18.7 kDa
포장
pkg of 10 μg
기술
cell culture | mammalian: suitable
불순물
<0.1 EU/μg endotoxin, tested
색상
white
UniProt 수납 번호
배송 상태
wet ice
저장 온도
−20°C
유전자 정보
mouse ... THPO(21832)
일반 설명
TPO (thrombopoietin) is a hematopoietic cytokine expressed in multiple tissue types. It is predominantly produced by liver and kidney and acts as a ligand for the cell surface receptor c-mpl.
TPO is a lineage specific growth factor, produced in the liver, kidney and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes, and promotes increased circulating levels of platelets in vivo. TPO signals through the c-mpl receptor and acts as an important regulator of circulating platelets. Human and murine TPO exhibits cross-species reactivity. Recombinant murine TPO is a fully biologically active 174 amino acid polypeptide (18.7 kDa), which contains the erythropoietin-like domain of the full length TPO protein.
생화학적/생리학적 작용
TPO (thrombopoietin) is the key regulator of thrombopoiesis for platelet lineage. In both mice and humans, inactivation of this gene results in significant decrease in number of mature platelets produced by marrow progenitors. Transgenic mice with increased expression of this protein show progressive fibrosis and osteosclerosis of the bone marrow, as well as histological changes in kidneys, leading to mesangioproliferative glomerulopathy.
서열
SPVAPACDPR LLNKLLRDSH LLHSRLSQCP DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT LNKF
물리적 형태
Lyophilized from 10 mM Sodium Phosphate, pH 8.0.
재구성
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of ?0.5 mg/ml. Slow to dissolve. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Bryan McIntosh et al.
Experimental hematology, 36(7), 799-806 (2008-04-16)
Platelet production is regulated primarily by the cytokine thrombopoietin (TPO). Although TPO is expressed in several different tissues, only in the bone marrow has the level of expression been reported to increase in response to reduced numbers of platelets. In
Haruko K Shimoda et al.
American journal of hematology, 82(9), 802-806 (2007-06-05)
We previously reported that mice transgenic (Tg) for thrombopoietin (TPO) developed progressive fibrosis and osteosclerosis of the bone marrow. Here, we show that TPO-overexpressing mice also exhibited notable histological changes in the kidneys, including an increased number of mesangial cells
Kuter, D.J., et al.
Cytokine Reference, 965-982 (2001)
Krasimira Rozenova et al.
Blood, 125(11), 1730-1738 (2015-02-01)
Hematopoietic stem cell (HSC) self-renewal and multilineage reconstitution are controlled by positive and negative signaling cues with perturbations leading to disease. Lnk is an essential signaling adaptor protein that dampens signaling by the cytokine thrombopoietin (Tpo) to limit HSC expansion.
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.