제품 SEQR은(는)귀하의 국가에서 현재 판매되지 않습니다. 고객지원팀으로 연락바랍니다.
추천 제품
기술
whole genome amplification: suitable
배송 상태
wet ice
저장 온도
−20°C
일반 설명
The SeqPlex™ RNA Amplification Kit for whole transcriptome amplification (WTA) is designed to facilitate next-generation sequencing (NGS) from small quantities or from degraded/highly fragmented RNA (e.g. RNA from formalin-fixed paraffin-embedded (FFPE) tissue samples). The SeqPlex kit allows the user to pre-amplify these and other small quantity/highly fragmented RNA samples for input into a NGS workflow. It also facilitates the amplification of non-polyA tailed RNA isolated from tissue, cultured cells, formalin-fixed samples, or serum while maintaining patterns of differential expression found in the unamplified sample.
특징 및 장점
- Low quantities of total RNA random priming technology amplifies fragmented or intact RNA from all sources including FFPE and RIP.
- Semi-degenerate library primer design for more complete transcriptome coverage and efficient priming.
- No need to fragment DNA before sequencing.
- Amplifies ds-cDNA in 8 hours or less.
- Compatible with all next generation sequencing platforms except Pacific Bioscience.
기타 정보
SEQR-500RXN is manufactured on-demand. Contact us for more information.
법적 정보
SeqPlex is a trademark of Sigma-Aldrich Co. LLC
관련 제품
제품 번호
설명
가격
Storage Class Code
11 - Combustible Solids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
DNA Sequencing Research Group (DSRG): Evaluation of RNA Amplification Kits at Subnanogram Input Amounts of Total RNA for RNA-Seq
Nicolet C, et al.
Journal of biomolecular techniques : JBT, 24, S70-S70 (2013)
Isaac Engel et al.
Nature immunology, 17(6), 728-739 (2016-04-19)
Natural killer T cells (NKT cells) have stimulatory or inhibitory effects on the immune response that can be attributed in part to the existence of functional subsets of NKT cells. These subsets have been characterized only on the basis of
Meritxell Jodar et al.
Human reproduction update, 19(6), 604-624 (2013-07-17)
BACKGROUND Spermatozoa are highly differentiated, transcriptionally inert cells characterized by a compact nucleus with minimal cytoplasm. Nevertheless they contain a suite of unique RNAs that are delivered to oocyte upon fertilization. They are likely integrated as part of many different
Giuliana P Mognol et al.
Proceedings of the National Academy of Sciences of the United States of America, 114(13), E2776-E2785 (2017-03-12)
T-cell exhaustion is a progressive loss of effector function and memory potential due to persistent antigen exposure, which occurs in chronic viral infections and cancer. Here we investigate the relation between gene expression and chromatin accessibility in CD8+ tumor-infiltrating lymphocytes
Kathryn D Tuttle et al.
Nature communications, 9(1), 2650-2650 (2018-07-10)
During development in the thymus, invariant natural killer T (iNKT) cells commit to one of three major functionally different subsets, iNKT1, iNKT2, and iNKT17. Here, we show that T cell antigen receptor (TCR) signal strength governs the development of iNKT
프로토콜
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
