추천 제품
Grade
for molecular biology
형태
buffered aqueous glycerol solution
농도
10,000 units/mL
배송 상태
wet ice
저장 온도
−20°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
특이성
Recognition sequence: 5′-GGTAC/C-3′
Cutting results: a 2-10-fold Kpn I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes.
Cutting results: a 2-10-fold Kpn I overdigestion of 1 μg λ DNA substrate results in 100% cutting
Heat inactivation: 65 °C for 15 minutes.
애플리케이션
KpnI is a DNA restriction enzyme that is used in molecular biology research to cleave DNA at the recognition sequence 5′-GGTAC/C-3′ to generate DNA fragments with 3′-cohesive termini.
기타 정보
Supplied with 10x Restriction Enzyme Buffer SL (B3782)
Comment: Kpn I shows abnormal DNA cleaving patterns (star activity) in reactions containing high (>5%) glycerol, >5% (v/v); excess enzyme and high pH (>8.0).
The enzyme will not cleave DNA containing 3′-residue of 5-methylcytosine.
The enzyme will not cleave DNA containing 3′-residue of 5-methylcytosine.
물리적 형태
Solution in 20 mM Tris-HCl, pH 7.5, 0.1 mM EDTA, 50 mM NaCl, 10 mM 2-mercaptoethanol, 50% glycerol (v/v), 0.01% polydocanol (v/v) at 4°C
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
J Tomassini et al.
Nucleic acids research, 5(11), 4055-4064 (1978-11-01)
We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Klebsiella pneumoniae. The enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the arrows, producing 3' protruding cohesive ends
T Welsch et al.
American journal of physiology. Renal physiology, 281(4), F769-F777 (2001-09-13)
Mice lacking the 80-kDa CD2-associated protein (CD2AP) develop progressive renal failure that starts soon after birth with proteinuria and foot process effacement by unknown mechanisms. CD2AP has been identified and cloned independently by virtue of its interaction with the T
Karel De Gendt et al.
Proceedings of the National Academy of Sciences of the United States of America, 101(5), 1327-1332 (2004-01-28)
Androgens control spermatogenesis, but germ cells themselves do not express a functional androgen receptor (AR). Androgen regulation is thought to be mediated by Sertoli and peritubular myoid cells, but their relative roles and the mechanisms involved remain largely unknown. Using
Jaroslav Jelinek et al.
Epigenetics, 7(12), 1368-1378 (2012-10-19)
Genome wide analysis of DNA methylation provides important information in a variety of diseases, including cancer. Here, we describe a simple method, Digital Restriction Enzyme Analysis of Methylation (DREAM), based on next generation sequencing analysis of methylation-specific signatures created by
Saqib H Ansari et al.
Journal of pediatric hematology/oncology, 35(4), e153-e156 (2013-02-08)
β-thalassemia is characterized by impaired β-chain synthesis leading to ineffective erythropoiesis, severe anemia, and a need for blood transfusion. Presence of Xmn I polymorphism (-158 C-T nucleotide change) in γ-globin gene is associated with a higher fetal hemoglobin and a
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