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Merck
모든 사진(1)

문서

P2192

Sigma-Aldrich

10X PCR Buffer

Optimized for routine PCR with MgCl2 included

동의어(들):

PCR Buffer Solution, PCR Reaction Buffer

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모든 사진(1)

About This Item

UNSPSC 코드:
41106306
NACRES:
NA.52

Quality Level

300

형태

liquid

포장

vial of 1.5 mL

색상

colorless

응용 분야

agriculture

외래 활성

DNase, RNase, none detected

저장 온도

−20°C

애플리케이션

10X PCR Buffer has been used as a component of the PCR mixture:
  • for the amplification of viral DNA
  • to amplify genomic DNA to study the methylenetetrahydrofolate reductase (MTHFR) gene mutations in attention deficit hyperactivity disorder (ADHD) individuals
  • to amplify 16S-23S rRNA gene internal transcribed spacer (ITS) from Bacillus sp.

특징 및 장점

  • Standalone buffer
  • Compatible with JumpStart™ Taq DNA Polymerase (D9307), Taq DNA Polymerase from Thermus aquaticus (D1806), and REDTaq® Genomic DNA Polymerase (D8312)

성분

Composition of the 10× buffer: 100 mM Tris-HCl, pH 8.3 at 25°C; 500 mM KCl; 15 mM MgCl2; 0.01% gelatin

법적 정보

JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Maike Stam et al.
Genetics, 162(2), 917-930 (2002-10-26)
Paramutation is an interaction between alleles that leads to a heritable change in the expression of one allele. In B'/B-I plants, B-I (high transcription) always changes to B' (low transcription). The new B' allele retains the low expression state in
Ákos Boros et al.
PloS one, 6(12), e29145-e29145 (2011-12-24)
A novel positive-sense, single-stranded RNA (+ssRNA) virus (Halastavi árva RNA virus, HalV; JN000306) with di-cistronic genome organization was serendipitously identified in intestinal contents of freshwater carps (Cyprinus carpio) fished by line-fishing from fishpond "Lőrinte halastó" located in Veszprém County, Hungary.
G J Breedveld et al.
Neurology, 59(4), 579-584 (2002-08-28)
Benign hereditary chorea (BHC) is an autosomal dominant disorder that can be distinguished from Huntington disease by its early onset, stable or only slightly progressive course, and absence of mental deterioration. The variation in clinical features is such that its
Phylogenetic Analysis of Baculovirus Isolates from Diseased Insects in Southern Vietnam
Thao N T P, et al.
Open Journal of Genetics, 2014 (2014)
Cem Gokcen et al.
International journal of medical sciences, 8(7), 523-528 (2011-09-08)
The purpose of this study was to evaluate the relationship between 5,10- methylenetetrahydrofolate reductase (MTHFR) polymorphisms and Attention Deficit Hyperactivity Disorder (ADHD) in a sample of Turkish children. MTHFR gene polymorphisms were assessed in 40 patients with ADHD and 30
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프로토콜

Amplification of DNA Using Jumpstart™ REDTaq® DNA Polymerase (D8187)

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

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