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Merck
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주요 문서

MAK193

Sigma-Aldrich

Citrate Synthase Activity Assay Kit

Citrate Synthase Activity Assay Kit

Sufficient for 100 Colorimetric tests

동의어(들):

Citrate Synthase Test Kit

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About This Item

EC Number:
UNSPSC 코드:
12161503
NACRES:
NA.84
가격 및 재고 정보를 현재 이용할 수 없음

검출 방법

colorimetric

관련 질환(들)

endocrinological disorders, diabetes; cancer

저장 온도

−20°C

유전자 정보

human ... CS(1431)
mouse ... CS(12974)
rat ... CS(170587)

일반 설명

Citrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern.[1]

애플리케이션

Citrate Synthase Activity Assay Kit has been used to measure the activity of the enzyme citrate synthase in samples.[2]

적합성

Suitable for the detection of citrate synthase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

원리

The Citrate Synthase Activity Assay kit provides a simple and direct procedure for measuring the activity of CS in a variety of tissues, cell cultures (adherent or suspension), and purified mitochondria. CS activity is determined using a coupled enzyme reaction, which results in a colorimetric (412 nm) product proportional to the enzymatic activity present. One unit of CS is the amount of enzyme that generates 1.0 mmole CoA per minute at 25 °C and pH 7.2.

키트 구성품 전용

제품 번호
설명

  • CS Assay Buffer

  • CS Substrate Mix

  • CS Developer

  • GSH Standard, Reduced

  • CS Positive Control

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Moritz A Niederschweiberer et al.
Frontiers in molecular biosciences, 8, 671274-671274 (2021-07-02)
Alzheimer's disease (AD), the most prevalent form of dementia, affects globally more than 30 million people suffering from cognitive deficits and neuropsychiatric symptoms. Substantial evidence for the involvement of mitochondrial dysfunction in the development and/or progression of AD has been shown
Tony Chao et al.
American journal of physiology. Cell physiology, 317(6), C1229-C1238 (2019-09-19)
Severe burn injury results in systemic disruption of metabolic regulations and impaired cardiac function. Restoration of hemodynamic homeostasis utilizing intravenous (IV) fluids is critical for acute care of the burn victim. However, the effects of burns and resuscitation on cardiomyocyte
Tomoaki Aoki et al.
Frontiers in medicine, 8, 666735-666735 (2021-04-30)
Background: Identification of the mechanisms underlying mitochondrial dysfunction is key to understanding the pathophysiology of acute injuries such as cardiac arrest (CA); however, effective methods for measurement of mitochondrial function associated with mitochondrial isolation have been debated for a long
Regulation of Expression of Citrate Synthase by the Retinoic Acid Receptor-Related Orphan Receptor α (RORα)
Crumbley C, et al.,
PLoS ONE, 7, e33804 -e33804 (2012)
P Dongil et al.
Scientific reports, 8(1), 13810-13810 (2018-09-16)
Metabolic dysfunction in the liver is the cause of numerous pathologies, which are associated with an altered redox state. PASK (PAS Domain Kinase) is a nutrient and bioenergetic sensor. We contend that PASK could act as an oxidative stress sensor

질문

  1. Is the sensitivity information for the CS0720 (Citrate Synthase Assay Kit) available?

    1 답변
    1. The lower concentration tested for the product CS0720 (Citrate Synthase Assay Kit) is 1u/µgP

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활성 필터

  1. USA
    • 상품평 1
    • 투표 0
    별 5개 중 1개입니다.

    This actually measures total deacetylation activity, not specific to CS

    The mechanism for the assay kit is the deacetylation of Acetyl-CoA to CoA. There are multiple enzymes can do this, not just CS. This assay kit doesn't have a good control for the background non-CS-related deacetylation step, so it is not the right product to measure CS activity. CS0720 is the CS specific assay kit to buy.

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    도움이 되었습니까?

    1. Sigma-Aldrich Corporation의 답변:

      MAK193 has been discontinued, CS0720 is its recommended replacement. CS0720 includes a positive control (which MAK193 lacked). CS0720 provides the Acetyl Coenzyme A and the Oxaloacetic acid needed for Citrate Synthase to produce Citrate.

      We encourage customers who experience problems with our products to contact us for additional technical support. Please visit https://www.sigmaaldrich.com/US/en/support/customer-support to submit a Product Technical Inquiry.

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