M1302

M-MLV Reverse Transcriptase

Moloney Murine Leukemia Virus enzyme & buffer for cDNA synthesis

• 동의어(들): Moloney Murine Leukemia Virus Reverse Transcriptase
• CAS Number: 9068-38-6
• MDL number: MFCD00283754
• UNSPSC 코드: 12352202
• NACRES: NA.55

속성

• 생물학적 소스: Porcine intestinal mucosa
• 재조합: expressed in E. coli
• 형태: liquid
• 사용: sufficient for 200 reactions; sufficient for 250 reactions
• 특징: dNTPs included: no; hotstart: no
• 농도: 200 units/μL
• 기술: RT-PCR: suitable
• 색상: colorless
• 입력: purified RNA
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

Moloney murine leukemia virus (M-MLV ) reverse transcriptase enzyme is isolated from E. coli expressing a portion of the pol gene of M-MLV on a plasmid. MoMLV RT is made up of 671 amino acid residues. It is a DNA polymerase that uses single-stranded RNA, DNA, or an RNA-DNA hybrid (using a primer) to synthesize a complementary DNA strand.

애플리케이션

M-MLV Reverse Transcriptase has been used:

• for the preparation of cDNA libraries or for first strand cDNA synthesis
• for use in a 2-step RT-PCR assay
• in quantitative realtime-polymerase chain reaction (RT-qPCR)
• in reverse transcription

특징 및 장점

• Thermostable reverse transcriptase active at 37 °C.
• Can be used to generate first strand cDNA of up to 7 kb.

포장

Supplied with 10× M-MLV reverse transcriptase buffer containing DTT.

단위 정의

One unit incorporates 1 nmol of TTP into acid precipitable material in 10 min. at 37 °C using poly(A):oligo dT as a template:primer.

제조 메모

The enzyme is purified from Escherichia coli expressing the pol gene of M-MLV on a plasmid.

법적 정보

Purchase of this product is accompanied by a limited license for use in the Polymerase Chain Reaction (PCR) process for research purposes only and in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by an up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., and authorized thermal cycler.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)