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Merck
모든 사진(1)

문서

L9267

Sigma-Aldrich

Lectin from Lens culinaris (lentil)

lyophilized powder

동의어(들):

Lens culinaris agglutinin, LcH

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About This Item

UNSPSC 코드:
12352202
NACRES:
NA.32

형태

lyophilized powder

효능

<16 μg per mL agglutination activity

불순물

salt, free

저장 온도

2-8°C

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일반 설명

Lectins are proteins of plant origin, which have the ability to bind to certain neuronal surface glycoproteins and glycolipids. This ability of theirs helps them to be used as neuroanatomical tracers, as they are taken and transported in a retrograde or anterograde fashion.

애플리케이션

Lectin from Lens culinaris (lentil) is used for size- or charge-based separation of proteins with the ANA. (Anisotropic Nanofilter Array: ANA).

생화학적/생리학적 작용

LcH is not blood group specific. It has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. LcH is comprised of two isomers, LcH-A (mol. wt. 49,000) and LcH-B (mol. wt. 49,000). LcH-A is mitogenic. Conjugates are prepared from purified lectin.

품질

Contains two major bands on electrophoresis corresponding to isolectins LcH-A and LcH-B.

제조 메모

Highly purified by affinity chromatography.

분석 메모

Agglutination activity is expressed in μg per mL and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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문서 라이브러리 방문

U K Nath et al.
TheScientificWorldJournal, 2014, 372405-372405 (2015-01-13)
Lentil is one of the most important pulse crops in the world as well as in Bangladesh. It is now considered a main component for training and body building practising in first world countries. Yield varies tremendously from year to
Jianping Fu et al.
Nature nanotechnology, 2(2), 121-128 (2008-07-26)
Microfabricated regular sieving structures hold great promise as an alternative to gels to improve the speed and resolution of biomolecule separation. In contrast to disordered porous gel networks, these regular structures also provide well defined environments ideal for the study
Oscar A Peralta et al.
Reproductive biology and endocrinology : RB&E, 11, 55-55 (2013-06-27)
The objectives of the study were to characterize the expression of the α- and β-subunits of granulocyte-macrophage colony stimulating factor (GM-CSF) receptor in bovine cumulus cells and oocytes and to determine the effect of exogenous GM-CSF on cumulus cells expansion
C R Gerfen et al.
Brain research, 290(2), 219-238 (1984-01-09)
A new neuroanatomical method for tracing connections in the central nervous system based on the anterograde axonal transport of the kidney bean lectin, Phaseolus vulgaris-leucoagglutinin (PHA-L) is described. The method, for which a detailed protocol is presented, offers several advantages
Anders Heebøll-Nielsen et al.
Biotechnology and bioengineering, 87(3), 311-323 (2004-07-29)
This work presents the development, testing, and application in high-gradient magnetic fishing of superparamagnetic supports for adsorption of lectins. Various approaches were examined to produce affinity, mixed mode, and hydrophobic charge induction type adsorbents. In clean monocomponent systems affinity supports

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