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포장
pack of 1 × 250 pieces
제조업체/상표
Cytiva 28-9042-70
일반 설명
PlasmidPrep Mini Spin Kit uses a simple plasmid DNA purification protocol involving a modified alkaline lysis procedure and a novel silica-based membrane to achieve highly efficient plasmid DNA purification.
The extracted plasmid DNA resulting from performing a miniprep is called a "plasmid miniprep" and is often performed using a plasmid purification kit. Minipreps are used in the process of molecular cloning to analyze bacterial clones. When bacteria are lysed under alkaline conditions, both chromosomal DNA and protein are denatured, but the plasmid DNA remains stable. On addition of acetate-containing neutralization buffer the larger and less supercoiled genomic DNA and proteins precipitate, but the small bacterial DNA plasmids stay in the solution.
The typical plasmid DNA yield from a fresh 1.5 mL culture of E. coli containing a high copy number plasmid is approximately 9 μg. Instead of organic solvents, the kit contains chaotropic salts to denature protein components and promote selective binding of DNA to the novel silica membrane.
The kit contains spin columns prepacked with a novel silica membrane, suspension, lysis, and neutralization buffers containing chaotropic salts, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.
The extracted plasmid DNA resulting from performing a miniprep is called a "plasmid miniprep" and is often performed using a plasmid purification kit. Minipreps are used in the process of molecular cloning to analyze bacterial clones. When bacteria are lysed under alkaline conditions, both chromosomal DNA and protein are denatured, but the plasmid DNA remains stable. On addition of acetate-containing neutralization buffer the larger and less supercoiled genomic DNA and proteins precipitate, but the small bacterial DNA plasmids stay in the solution.
The typical plasmid DNA yield from a fresh 1.5 mL culture of E. coli containing a high copy number plasmid is approximately 9 μg. Instead of organic solvents, the kit contains chaotropic salts to denature protein components and promote selective binding of DNA to the novel silica membrane.
The kit contains spin columns prepacked with a novel silica membrane, suspension, lysis, and neutralization buffers containing chaotropic salts, wash and elution buffers, microcentrifuge collection tubes, a full protocol booklet, and a detachable, quick reference protocol card.
특징 및 장점
- Produces high-quality plasmid DNA with excellent reproducibility for use in cloning, restriction enzyme digestion, PCR amplification, and DNA sequencing.
- Total preparation time of less than 10 min represents a 50% reduction in protocol time
- Increased ease of use provided by minimal changes in pipetting volume from one part of the protocol to the next
- Fewer centrifugation stepsColor-coded tubes and columns simplify the protocol
- No organic solvents such as phenol chloroform
저장 및 안정성
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
분석 메모
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
법적 정보
illustra is a trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Storage Class Code
8B - Non-combustible corrosive hazardous materials
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
문서
This page discusses methods for plasmid DNA purification and the factors impacting resulting DNA purity and yield using illustra plasmidPrep Kits from Cytiva.
This page shows possible causes and solutions for problems that may occur during plasmid DNA preparation.
프로토콜
This page shows Plasmid DNA purification using illustra™ plasmidPrep Mini Spin Kit from Cytiva.
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