SK-N-BE(2)

95011815, human nerve, Epitheilioid & neuroblastoid

• UNSPSC 코드: 41106514

속성

• product name: SK-N-BE(2), 95011815
• 생물학적 소스: human nerve
• 성장 모드: Adherent
• 핵형: Modal no. 44
• 형태학: Epitheilioid & neuroblastoid
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): metastasis
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian neuroblastoma

세포주 설명

Established from a bone marrow biopsy of a 22-month-old male with disseminated neuroblastoma in 1972. The morphology varies with sub-population from cells with long processes through small, flattened, epithelioid cells. Dense cultures tend to form loosely adherent aggregates. They are multipotential with regard to neuronal enzyme expression and display a high capacity to convert tyrosine to dopamine.

애플리케이션

Chemotherapy development, chromosome studies, drug resistance, differentiation studies.

배양 배지

EMEM (EBSS) + 1% Non Essential Amino Acids (NEAA) : Ham′s F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:10 to 1:25 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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