A9(Hamprecht)

8062526, mouse connective tissue, Fibroblast

• UNSPSC 코드: 41106514

속성

• product name: A9(Hamprecht), 08062526
• 생물학적 소스: mouse connective tissue
• 성장 모드: Adherent
• 핵형: Modal No of chromosomes is 54
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse Fibroblast (L cells)

세포주 설명

A 6-thioguanine resistant mutant of mouse L cell fibroblasts (Mouse strain C3H/An). A9 lacks guanylic acid-inosinic acid pyrophosphorylase and was isolated by virtue of resistance to 6-thioguanine (3 μg/ml). ECACC has two catalogue entries for the A9 cell line which differ in that they were deposited by different groups: A9 (Sigma Catalogue number 85011426) and A9(Hamprecht) (Sigma Catalogue number 08062526). Several other cell lines in the ECACC catalogue have been derived from A9(Hamprecht).

애플리케이션

Used in the generation of cell hybrids.

배양 배지

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + 0.1 mM 6-thioguanine

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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