A9207

Avidin−Agarose from egg white

aqueous glycerol suspension

• 동의어(들): Avidin agarose
• MDL number: MFCD00130572
• UNSPSC 코드: 41106514
• NACRES: NA.56

속성

• 생물학적 소스: egg white
• 결합: agarose conjugate
• 형태: aqueous glycerol suspension
• 기질: cross-linked 6% beaded agarose
• 기질 활성: epichlorohydrin
• 기질 부착: amino
• 기질 스페이서: 15 atoms
• 저장 온도: −20°C

설명

일반 설명

Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.

애플리케이션

Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.

Avidin-Agarose from egg white has been used:

• in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
• in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
• in avidin bead assay for purification of labelled biotin
• for capture of glycoproteins from murine cornea samples

생화학적/생리학적 작용

Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.

단위 정의

One unit will bind 1.0 μg of d-biotin.

물리적 형태

Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable