94403

Hoechst 33258 solution

1 mg/mL in H₂O, ≥98.0% (HPLC)

• 동의어(들): bisBenzimide H 33258 solution
• 실험식(Hill 표기법): C₂₅H₂₄N₆O · 3HCl
• CAS Number: 23491-45-4
• Molecular Weight: 533.88
• UNSPSC 코드: 12171500
• PubChem Substance ID: 329770334
• NACRES: NA.32

속성

• 분석: ≥98.0% (HPLC)
• 형태: liquid
• 농도: 1 mg/mL in H₂O
• solubility: DMF: soluble; H₂O: soluble
• 형광: λ_ex 355 nm; λ_em 465 nm in TE buffer; DNA
• 저장 온도: 2-8°C
• SMILES string: Cl.Cl.Cl.CN1CCN(CC1)c2ccc3[nH]c(nc3c2)-c4ccc5nc([nH]c5c4)-c6ccc(O)cc6
• InChI: 1S/C25H24N6O.3ClH/c1-30-10-12-31(13-11-30)18-5-9-21-23(15-18)29-25(27-21)17-4-8-20-22(14-17)28-24(26-20)16-2-6-19(32)7-3-16;;;/h2-9,14-15,32H,10-13H2,1H3,(H,26,28)(H,27,29);3*1H
• InChI key: SMNPLAKEGAEPJD-UHFFFAOYSA-N

설명

일반 설명

Hoechst 33258, also known as bisbenzimide, is a fluorescent blue dye. It is categorized as a chromosomal stain that binds to the minor groove of the DNA double helix recognizing a run of four A-T base pairs.

애플리케이션

Hoechst 33258 is used to estimate DNA concentration in samples by fluorometry which is better than spectrophotometry, allowing the determination of nanogram quantities of DNA. Also, Hoechst 33258 is suitable to detect mycoplasma contamination in cell cultures due to its high fluorescence ability.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable