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문서

67996

Sigma-Aldrich

Tris Acetate-EDTA buffer Concentrate

for Northern and Southern blotting, solution

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About This Item

UNSPSC 코드:
12161700
NACRES:
NA.25

Grade

for molecular biology

형태

solution

품질

50x Concentrate

불순물

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH

8.3±0.2 (25 °C)

density

1.09 g/mL at 20 °C

저장 온도

room temp

일반 설명

TBE (Tris/Acetate/EDTA) Buffer is commonly used in nucleic acid electrophoresis. This solution has a lower buffering capacity than TBE buffer, but double stranded DNA runs faster with TAE buffer. This 50x concentrate can be easily diluted with molecular biology grade water to 1x before use.

애플리케이션

Tris Acetate-EDTA buffer concentrate is suitable for use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures. It is especially useful for double stranded DNA separations.

성분

The TAE 50x liquid concentrate contains 2M TRIS acetate and 0.05M EDTA in water.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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