MABF2752

Anti-HHV-8 PF-8 Antibody, clone 11D1

• 동의어(들): PF-8;KSHV ORF59
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified antibody
• 항체 생산 유형: primary antibodies
• 클론: 11D1, monoclonal
• 분자량: calculated mol wt 42.41 kDa
• 정제법: using protein G
• 종 반응성: virus
• 포장: antibody small pack of 100 μg
• 기술: immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• 동형: IgG2b
• 에피토프 서열: Unknown
• 단백질 ID 수납 번호: Unknown
• UniProt 수납 번호: A0A0N9RMW6
• 배송 상태: ambient
• 타겟 번역 후 변형: unmodified
• 유전자 정보: vaccinia virus ... ORF45(4961474)

설명

일반 설명

Human herpesvirus-8 (also known as Kaposi′s sarcoma-associated herpesvirus, HHV-8, KSHV) is etiologically associated with all forms of Kaposi sarcoma (KS) including classic, endemic, transplant-related, and AIDS-related, as well as rare neoplastic disorders. It is a double stranded DNA virus with a large genome that encodes for over 87 open reading frames (ORFs) including genes necessary for capsid, tegument, envelope, DNA replication and regulatory proteins. HHV-8 undergoes a bi-phasic lifecycle and can persist indefinitely in the infected host in a latent form during which time only a small fraction of regulatory viral proteins is expressed. The most prominent protein expressed in latently infected cells is the ORF73 gene product known as the latency-associated nuclear antigen (LANA) that functions to ensure the maintenance of the viral genome by tethering the viral episomal DNA to host cell chromosomes. The ORF59 (also known as PF-8), a phosphoprotein, is reported to be a processivity factor for HHV-8 DNA polymerase (Pol-8). It interacts with and stimulates Pol-8 DNA synthesis activity. It is an early-late gene product localized to HHV-8-infected cell nuclei. PF-8 interacts with HHV-8 DNA polymerase (Pol-8) and stimulates Pol-8 DNA synthesis activity. Amino acid residues 10-27 and 279-301 are reported to be critical for the processivity function of PF-8. Residues 10 to 27 are essential for its interaction with Pol-8 and 279-301 are required for its binding to dsDNA. Clone 11D1 specifically detects PF-8 of HHV-8 and exhibits predominant nuclear membrane fluorescence in TPA (phorbol ester)-induced, HHV-infected BCBL-1 cells. (Ref.: Strahan, RC., et al. (2017). PLoS Pathog 13(7); e1006482; Chan, SR., and Chandran, B (2000). J. Virol. 74(23); 10920-10929; Chan, SR., et al. (1998). 240: 118-126).

특이성

Clone 11D1 is a mouse monoclonal antibody that detects PF-8 of Kaposi′s sarcoma-associated herpesvirus (HHV-8).

면역원

12-O-Tetradecanoylphorbol 13-acetate (TPA)-induced BCBL-1 cells.

애플리케이션

Quality Control Testing

Evaluated by Immunocytochemistry in BCBL-1 cells.

Immunocytochemistry Analysis (ICC): A 1:50 dilution of this antibody detected Kaposi s sarcoma associated herpesvirus (HHV-8) in 12-O-Tetradecanoylphorbol 13-acetate (TPA) treated (20 ng/mL; 48 h) BCBL-1 cells.

Tested Applications

Immunoprecipitation Analysis: A representative lot immunoprecipitated PF-8 of HHV-8 in Immunoprecipitation applications (Chan, S.R., et al. (1998). Virology. 240(1):118-26; Chan, S.R., et al. (2000). J Virol. 74(23):10920-9).

Western Blotting Analysis: A representative lot detected OF-8 of HHV-8 in Western Blotting applications (Chan, S.R., et al. (1998). Virology.;240(1):118-26; Chan, S.R., et al. (2000). J Virol.;74(23):10920-9).

Immunocytochemistry Analysis: A representative lot detected PF-8 of HHV-8 in Immunocytochemistry applications (Chan, S.R., et al. (1998). Virology.;240(1):118-26).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-HHV-8 PF-8, clone 11D1, Cat. No. MABF2752, is a mouse monoclonal antibody that detects PF-8 of Kaposi′s sarcoma-associated herpesvirus (HHV-8) and is tested for use in Immunocytochemistry, Immunoprecipitation, and Western Blotting.

물리적 형태

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Recommended storage: +2°C to +8°C.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable