콘텐츠로 건너뛰기
Merck
모든 사진(1)

Key Documents

MAB1905-I

Sigma-Aldrich

Anti-Laminin α1/β1 Antibody, clone AL-4

clone AL-4, from rat

동의어(들):

Laminin subunit beta-1, Laminin subunit alpha-1, Laminin α1/β1, Serine/threonine-protein kinase PLK4

로그인조직 및 계약 가격 보기


About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rat

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

AL-4, monoclonal

종 반응성

mouse

기술

immunocytochemistry: suitable
western blot: suitable

동형

IgG2aκ

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

mouse ... Lama1(16772)

일반 설명

Laminins are secreted glycoproteins, and are an important structural component of the extracellular matrix. The family of laminins is comprised of multiple isoforms, each formed from the oligomerization of various alpha, beta, and gamma chains. Laminin isoforms are expressed in a tissue-specific pattern, and contain multiple domains, which may include laminin EGF-like domains; laminin G-domains; laminin IV type A domains; and a laminin N-terminal domain. Laminins are capable of self-assembly to form a structural scaffold; they also bind to cell-surface molecules such as dystroglycan and mediate attachment of these molecules to the extracellular matrix. Laminins may also participate in intracellular signaling processes by binding to various integrin receptors. They are involved in cell adhesion, migration, differentiation, neurite outgrowth, and metastasis.

특이성

As originally characterized, clone AL-4 reacts with EHS mouse laminin, A chain (laminin α1). The epitope is mapped to the carboxy terminal globular domain on the A chain. Recent work by Scheele, et al. (2006) has further demonstrated that clone AL-4, under reducing conditions, recognized both the 200 kDa β1/γ1 band and the 400 kDa α1 band of mouse EHS laminin. However the affinity for the 400 kDa band was very low. AL-4 also demonstrated strong binding the Laminin β1. By ELISA the epitope for the AL-4 seemed to be located on the Laminin β1 chain, however the clone also recognizes the laminin α1 chain in immunoblots, thus AL-4 seems to show mixed cross reactivity to laminins α1 and β1, with the β1 reactivity being more prominent.

면역원

Laminin isolated from mouse EHS tumor

애플리케이션

Anti-Laminin α1/β1 Antibody, clone AL-4 is an antibody against Laminin α1/β1 for use in Western Blotting, ICC.
Research Category
Cell Structure
Research Sub Category
ECM Proteins

품질

Evaluated by Western Blot in Laminin purified protein.

Western Blot Analysis: 0.25 µg/mL of this antibody detected Laminin α1/β1 in 10 µg of Laminin purified protein.

표적 설명

~170 kDa observed

결합

Replaces: MAB1905

물리적 형태

Format: Purified
Protein G Purified
Purified rat monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
Laminin purified protein

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

적합한 제품을 찾을 수 없으신가요?  

당사의 제품 선택기 도구.을(를) 시도해 보세요.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Neena Lala-Tabbert et al.
Skeletal muscle, 6(1), 40-40 (2016-12-08)
Postnatal growth and repair of skeletal muscle relies upon a population of quiescent muscle precursor cells, called satellite cells that can be activated to proliferate and differentiate into new myofibers, as well as self-renew to replenish the satellite cell population.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.