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Merck
모든 사진(5)

Key Documents

ABE48

Sigma-Aldrich

Anti-NELF-E Antibody

from rabbit, purified by affinity chromatography

동의어(들):

Negative elongation factor E, NELF-E, RNA-binding protein RD

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

rat, human, mouse

종 반응성(상동성에 의해 예측)

canine (based on 100% sequence homology), bovine (based on 100% sequence homology)

기술

immunocytochemistry: suitable
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... NELFE(7936)

일반 설명

NELF-E (Negative elongation factor E) is a main component of NELF human transcription factor complex which associates with DRB sensitivity-inducing factor to stifle transcriptional elongation by RNA polymerase II. NELF-E is an RNA-binding protein that includes arginine-aspartic acid dipeptide repeats. NELF-E RNA-binding activity is essential for NELF to function.

면역원

KLH-conjugated linear peptide corresponding to human NELF-E.

애플리케이션

Anti-NELF-E Antibody is a Rabbit Polyclonal Antibody for detection of NELF-E also known as Negative elongation factor E, NELF-E, RNA-binding protein RD & has been validated in ICC & WB.
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected NELF-E in NIH/3T3 and HeLa cells.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

품질

Evaluated by Western Blot in L6 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NELF-E on 10 µg of L6 cell lysate.

표적 설명

~ 43 kDa observed. Uncharacterized bands may be due to post-translational modification.

물리적 형태

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
L6 cell lysate

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Integrator complex regulates NELF-mediated RNA polymerase II pause/release and processivity at coding genes.
Stadelmayer, B; Micas, G; Gamot, A; Martin, P; Malirat, N; Koval, S; Raffel, R; Sobhian et al.
Nature Communications null
Seychelle M Vos et al.
eLife, 5 (2016-06-11)
Transcription regulation in metazoans often involves promoter-proximal pausing of RNA polymerase (Pol) II, which requires the 4-subunit negative elongation factor (NELF). Here we discern the functional architecture of human NELF through X-ray crystallography, protein crosslinking, biochemical assays, and RNA crosslinking

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