ABE1943

Anti-HOXB13 Antibody

from rabbit, purified by affinity chromatography

• 동의어(들): Homeobox protein Hox-B13, HOXB13
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: human
• 기술: immunocytochemistry: suitable; immunohistochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_006352
• UniProt 수납 번호: Q92826
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HOXB13(10481)

설명

일반 설명

Homeobox protein Hox-B13 (UniProt Q92826) is encoded by the HOXB13 (also known as PSGD) gene (Gene ID 10481) in human. HOXB13 is a homeobox gene family member whose expression is restricted to prostate tissues and is highly correlated with that of androgen receptor (AR). Hox-B13 suppresses androgen-stimulated AR activity via direct AR interaction and thereby inhibits androgen-dependent (AD) prostate cancer (PCa) growth under androgen-rich conditions. On the other hand, HOXB13 is also known to be over-expressed in castration-resistant PCa (CRPC), where it plays a dichotomic role in promoting androgen-independent (AI) PCa growth. The zinc level in human prostate is markedly diminished in prostatic epithelial tumors and continues to decline during progression towards AI growth. HOXB13 over-expression upregulates transcription of its target genes SLC30A10 (ZnT10) and SLC30A4 (ZnT4) for zinc output transporters, which are responsible for the reduction in zinc level in CRPC. The diminished intracellular zinc level in turn stimulates NF-κB signaling and promotes invasion and metastasis of CRPC cells.

특이성

This polyclonal antibody selectively reacts with human HOXB13, but not the homologous HOXA13 or HOXD13.

면역원

KLH-conjugated linear peptide corresponding to the C-terminus of human HOXB13.

애플리케이션

This Anti-HOXB13 Antibody is validated for use in Western Blotting, Immunocytochemistry and Immunohistochemistry for the detection of HOXB13.

Western Blotting Analysis: A representative lot detected HOXB13 expression in human prostate cancer cell lines MDA PCa 2b, LNCaP, and C4-2, but not in prostate cancer DU145, PC3, CWR22Rv-1, or the non-tumorigenic prostate epithelial line P69 (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124).
Immunocytochemistry Analysis: A representative lot detected HOXB13 nuclear expression in MDA PCa 2b and LNCaP, but not PC3 or DU145, human prostate cancer cell lines (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124).
Immunohistochemistry Analysis: A representative lot detected differential HOXB13 expressions among various androgen-dependent (AD) and androgen-independent (AI) human prostate cancer tissues (Kim, Y.R., et. al. (2010). Mol Cancer. 9:124; Kim, Y.R., et. al. (2014). Oncogene. 33(37):4558-4567).

품질

Evaluated by Western Blotting in LNCaP cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected HOXB13 in 10 µg of LNCaP cell lysate.

표적 설명

~35 kDa observed

물리적 형태

Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-HCI, pH 8.0, and 0.1% soidum azide.

기타 정보

Concentration: Please refer to lot specific datasheet.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable