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Merck
모든 사진(4)

문서

ABC12

Sigma-Aldrich

Anti-Bak (NT) Antibody

from rabbit, purified by affinity chromatography

동의어(들):

Apoptosis regulator BAK, BCL2-antagonist/killer 1, BCL2-like 7 protein, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, pro-apoptotic protein BAK

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

affinity isolated antibody

항체 생산 유형

primary antibodies

클론

polyclonal

정제법

affinity chromatography

종 반응성

mouse, rat, human

기술

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... BAK1(578)

일반 설명

Bak or Bcl2 homologous antagonist is a member of the Bcl2 family of proteins. The Bcl-2 related proteins interact with one another through the formation of homo and heterodimers. The susceptibility of cells to apoptotic stimuli is thought to be controlled by the relative ratios of the different Bcl2 family proteins. Bak has been demonstrated to accelerate the rate of apoptosis in growth factor deprived murine lymphoid, neuronal and fibroblastic cell lines. This protein localizes to mitochondria, and functions to induce apoptosis. It interacts with and accelerates the opening of the mitochondrial voltage-dependent anion channel, which leads to a loss in membrane potential and the release of cytochrome c. Bak also interacts with the tumor suppressor P53 after exposure to cell stress.

특이성

This antibody recognizes BAK.

면역원

KLH-conjugated linear peptide corresponding to human BAK at and around the N-terminus.

애플리케이션

Detect Bak (N-terminus) using this Anti-Bak (N-terminus) Antibody validated for use in WB, IH, IC & IP.
Western Blot (SNAP ID) Analysis: 1 - 4 µg/ml from a previous lot detected Bak on 10 µg of Hek293 cell lysate.

Immunoprecipitation Analysis: For unpurified antibodies. 10 µg from a previous lot immunoprecipitated Bak from 500 µg of NIH3T3 lysate.

Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected Bak in kidney tissue.

Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected Bak in HeLa, NIH/3T3, and A431 cells.

품질

Evaluated by Western Blot in Hek293 cell lysate.

Western Blot Analysis: 0.5 µg/ml of this antibody detected Bak on 10 µg of Hek293 cell lysate.

표적 설명

~ 24 kDa

결합

Replaces: 04-433

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Lindsey M Ludwig et al.
Methods in molecular biology (Clifton, N.J.), 1877, 77-91 (2018-12-12)
The BCL-2 family of proteins orchestrates a complex signaling network that governs the balance between cellular survival and death. A comprehensive understanding of the mechanistic interactions between these proteins continues to evolve in normal and malignant cells. The functional variation
Yonghua Zhuang et al.
Journal of virology, 90(17), 7684-7691 (2016-06-17)
The tumor suppressor p53 plays a critical part in determining cell fate both as a regulator of the transcription of several proapoptotic genes and through its binding interactions with Bcl-2 family proteins at mitochondria. We now demonstrate that p53 protein
Barbara Pernaute et al.
Developmental cell, 57(11), 1316-1330 (2022-05-22)
The changes that drive differentiation facilitate the emergence of abnormal cells that need to be removed before they contribute to further development or the germline. Consequently, in mice in the lead-up to gastrulation, ∼35% of embryonic cells are eliminated. This

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